Description

Precast-GLgel Hepes Native-PAGE 10% (10-Well) is a ready-to-use, non-denaturing gel designed for the separation of proteins in their native, functional state. Made with an innovative glass plate construction, it significantly reduces non-specific protein adsorption, producing clearer and sharper native protein bands.

This 10% HEPES-based precast gel is supplied fully prepared along with matching Native-PAGE running buffer, allowing users to directly load samples without preparing their own gel. Its 10% acrylamide concentration provides excellent resolving performance for medium to large molecular weight native proteins, and is highly suitable for analyzing membrane proteins, membrane protein complexes, and native oligomeric protein assemblies that must maintain biological conformation during electrophoresis.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 10% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 ¡Á 7.4 ¡Á 0.15 cm
Plate size	9.8 ¡Á 8.4 ¡Á 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 ¦ÌL
Recommended sample loading volume	30 ¦ÌL

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Buffers

Buffer	Hepes Running Buffer for Native PAGE (Powder)
Specification	10*500 mL
Introduction	Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1¡Á working solution.
Protocol (for reference only)	Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely. Make up to 500 mL in the graduated cylinder with distilled water, which is 1¡Á working solution and can be used after mixing. Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4¡ãC.
Notes	1¡Á working buffer should not be used for more than 2 weeks after preparation. This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries. For research use only. Not for use in diagnostic procedures. For your safety and health, please wear personal protective equipment and clothing when operating.
Storage	Store at room temperature, valid for 2 years.

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Features

Glass plate construction effectively minimizes non-specific protein adsorption, resulting in clearer and sharper native protein bands.
Automatic gel filling technology ensures outstanding batch-to-batch stability and reproducibility.
10% gel concentration provides ideal resolution for medium to large molecular weight native proteins.
Highly suitable for membrane proteins and membrane protein complexes, which require mild non-denaturing conditions for structural preservation.
Supports electrophoresis of native protein complexes, including dimers, oligomers, and multi-subunit assemblies.
SDS-free matrix, specifically designed for true non-denaturing Native-PAGE experiments.
Ready-to-use format, eliminates manual gel preparation and saves significant experimental time.
Long shelf life, conveniently stored at 2¨C8¡ãC.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of medium to large molecular weight native proteins
Electrophoresis of membrane proteins and membrane protein complexes
Analysis of native oligomers and protein complexes
Western blot (WB) after native separation
Experiments requiring a 10% native gel

Storage

2~8¡ãC, do not freeze

Precast-Gel Hepes-Tris Native-PAGE 10% 10Well