Description

Precast-GLgel Hepes Native-PAGE 10% (10-Well) is a ready-to-use, non-denaturing gel designed for the separation of proteins in their native, functional state. Made with an innovative glass plate construction, it significantly reduces non-specific protein adsorption, producing clearer and sharper native protein bands.

This 10% HEPES-based precast gel is supplied fully prepared along with matching Native-PAGE running buffer, allowing users to directly load samples without preparing their own gel. Its 10% acrylamide concentration provides excellent resolving performance for medium to large molecular weight native proteins, and is highly suitable for analyzing membrane proteins, membrane protein complexes, and native oligomeric protein assemblies that must maintain biological conformation during electrophoresis.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 10% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Features

Glass plate construction effectively minimizes non-specific protein adsorption, resulting in clearer and sharper native protein bands.
Automatic gel filling technology ensures outstanding batch-to-batch stability and reproducibility.
10% gel concentration provides ideal resolution for medium to large molecular weight native proteins.
Highly suitable for membrane proteins and membrane protein complexes, which require mild non-denaturing conditions for structural preservation.
Supports electrophoresis of native protein complexes, including dimers, oligomers, and multi-subunit assemblies.
SDS-free matrix, specifically designed for true non-denaturing Native-PAGE experiments.
Ready-to-use format, eliminates manual gel preparation and saves significant experimental time.
Long shelf life, conveniently stored at 2–8°C.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of medium to large molecular weight native proteins
Electrophoresis of membrane proteins and membrane protein complexes
Analysis of native oligomers and protein complexes
Western blot (WB) after native separation
Experiments requiring a 10% native gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes Native-PAGE 10% 10Well