Description

Precast-Gel Hepes-Tris 4-20% (15-Well) is a high-performance precast polyacrylamide gel designed to deliver superior band resolution and consistent electrophoresis results. Manufactured using automated gel-casting technology, it ensures excellent stability and reproducibility. The glass-plate structure minimizes non-specific protein adsorption, resulting in sharper, clearer protein bands.

This gradient gel requires no SDS, making it compatible with both denaturing and native electrophoresis. It supports fast electrophoresis—typically completed within 40–50 minutes at 150 V—and fits most mainstream mini electrophoresis tanks. The gel cassette can be easily opened using a Gel Knife for convenient downstream analysis.

Specification

Height of 4% stacking gel	1.5 cm
Ratio of acrylamide to methylene bis acrylamide	29:1
Gel thickness	1.5 mm
Well format	10 wells / 15 wells
Max sample loading volume	30 μL/well for 15 wells; 60 μL/well for 10 wells
Cassette size	98 × 84 × 4.1 mm
Gel size	81 × 74 × 1.5 mm
Use	For research use only. Not for diagnostic procedures.

Features

High-performance precast polyacrylamide gel with improved band resolution.
Automated gel-casting technology ensures consistent stability and repeatability.
Glass-plate material reduces non-specific protein adsorption, producing sharper bands.
Electrophoresis can be completed in 40–50 minutes at 150 V.
Gel cassette opens easily using the Gel Knife—simply push gently along each side.
SDS-free design; compatible with both denaturing and native electrophoresis.
Fits most mainstream mini electrophoresis tanks, including Bio-Rad, Tanon, and Junyi Dongfang.
Available in multiple acrylamide concentrations: 6%, 8%, 10%, 12%, 15%, 4-15%, and 4-20%; custom concentrations available.
Compatible with fluorescent loading buffer Quick Bands (Catalog #: ES008) for direct band visualization under UV or LED light without staining or destaining.

Applications

Native-PAGE and denaturing PAGE experiments
Protein separation and analysis
Western blot sample preparation
Fluorescent protein visualization when used with Quick Bands loading buffer
Research laboratories requiring fast, high-resolution electrophoresis
Suitable for the separation and analysis of high–molecular-weight proteins and membrane protein complexes, which benefit from the gel’s stable structure and reduced non-specific adsorption.

Storage

Store at 4℃ for 18 months; do not freeze.

Precast-Gel Hepes-Tris 4-20% 15Well