Product overview

Biofargo precast, pre-stained agarose gels with GelRed® DNA gel stain are designed for fast, convenient, and safer DNA electrophoresis. Each mini-gel is factory cast in TAE with GelRed® premixed—no pouring, no staining, no post-stain. Just load your samples and run.

What you get

Every 10-gel pack includes free DNA loading buffer, your choice of DNA marker (ladder), and TAE instant granules (2 sticks, makes 2 L). Gels are available in 0.8–2.0% agarose (custom 0.75–2.5% on request) and multiple well counts to fit common mini gel chambers.

Ideal for

PCR product analysis
Restriction digest checks
Plasmid prep verification
DNA fragment sizing and cloning workflows

Features & Benefits

Ready-to-use & fast — Precast in TAE with GelRed®; no pouring, cooling, staining, or post-stain steps—unwrap, load, run.
High sensitivity, sharp bands — Optimized formula delivers clear, low-background signals; detect low-ng DNA with confidence.
Low sample input — Reliable visualization with small loading volumes.
Safer analysis — Uses GelRed® (Biotium), a safer alternative to EtBr.
Complete bundle — Includes DNA loading buffer, DNA marker (ladder), and 2 L TAE powder.
Multiple formats — Choose agarose 0.8–2.0% (custom 0.75–2.5%) and various well counts to fit your workflow.
Broad compatibility — Works with most mini electrophoresis chambers (standard mini-gel footprint).
Consistent results — Automated large-batch manufacturing ensures stable, reliable performance and consistent migration; lots are trackable for quality control.

Kit Components

10 × Precast agarose gels (1.2% Agarose, TAE, pre-stained with GelRed®; your selected well count—combs pre-installed)
1 × DNA loading buffer
1 × DNA marker (ladder) of your choice
2 × TAE buffer powder sticks (makes 2 L total)

Pack size: 10 gels/box

Customization:
We can manufacture precast gels at 0.75–2.5% agarose and in non-standard sizes to your specifications. For details, email contact@biofargo.com or call (804) 529-2296.

Shipping and Storage

Precast Gel Kit: 2–8 °C · Shelf life: 6 months
6× DNA Loading Buffer: 2–8 °C (short term) / −20 °C (long term) · Shelf life: 12 months
TAE Instant Granules: RT (15–25 °C) or −20 °C · Shelf life: 24 months
DNA Marker (Ladder): −20 °C (long term; 2–8 °C short term OK) · Shelf life: 12 months

Avoid repeated freeze–thaw for the loading buffer and marker; aliquot after first use.

Usage Notes

Higher sensitivity: GelRed® (high-molecular-weight dye) delivers up to ~8× the sensitivity of EtBr (depends on gel/imaging setup), allowing lower sample loads and sharper bands.
Routine checks: For Biofargo pre-stained gels, load 50–100 ng DNA per lane or 1–2 µL PCR product.
Unknown concentration: Start with ~1/5 to 1/3 of the DNA you would load on an EtBr gel; adjust based on signal.
When loading more DNA (e.g., for gel extraction):
- Run at lower voltage to reduce band distortion/saturation.
- After excision, fully dissolve the gel (extend melting time as needed) to help ensure high recovery.
Avoid repeated freeze–thaw of samples; if bands saturate, reduce load or exposure time.

Documents

Prestained Agarose Gel Electrophoresis Kit.pdf

Precast Agarose Gel, 1.2% agarose, with GelRed® Nucleic Acid Gel Stain (10000X), with TAE