Description

Porcine Primary Hepatocytes are derived from the the American Landrace Swine liver. Cells are freshly isolated and shipped in 10-50ml cell culture medium overnight at 4°C. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Porcine Primary Hepatocytes are tested for expression of markers using antibodies, ZO-1 by Immunofluorescence Staining. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged.

Suggested Medium

Hepatocyte Medium /w Kit (125 ml)

Catalog No. M1365

Laboratory Applications

Porcine Primary Hepatocytes can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, metabolism, drug-drug interaction, drug transporter activity toxicity of drug candidates or cell derivatives for desired research applications.

Cell Biologics' Cell Genotype Promise

Cell Biologics has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). Cell Biologics' cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.

Documents

P-6224

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Porcine Primary Hepatocytes - Plateable