Description

Biofargo PolyPeptone (G258) is a premium, specialized mixture of Casein Peptone and Meat Peptone designed as a superior nutrient source for general culture media formulation, delivering optimized microbial growth performance compared to individual peptones.

Specifications

Chemical / Physical Properties	Specifications	Typical Analysis
Amino Nitrogen (AN)	Minimum 3.7%	4.1%
Total Nitrogen (TN)	Minimum 10.0%	13.12%
AN / TN Ratio	N/A	31.25%
Loss on Drying	Maximum 6.0%	3.4%
Ash	Maximum 15.0%	8.8%
pH (2% Solution)	6.5 - 7.5	6.8
Elemental Profile & Microbial Limits
Calcium / Magnesium	N/A	Ca: 0.03% / Mg: 0.014%
Potassium / Sodium	N/A	K: 1.6% / Na: 2.12%
Standard Plate Count	< 5000 CFU/g	Complies
Yeast & Molds	< 100 CFU/g	Complies
Coliforms & Salmonella	Negative	Negative
Peptone Agar Growth Test	Satisfactory	Satisfactory

View Detailed Amino Acid Profile (g/100g)

Amino Acid	g/100g	Amino Acid	g/100g
Alanine	4.05	Lysine	5.5
Arginine	3.76	Methionine	1.81
Aspartic acid	8.83	Phenylalanine	3.34
Cystine	0.43	Proline	8.21
Glutamic acid	15.97	Serine	4.37
Glycine	5.7	Threonine	3.31
Histidine	1.81	Tryptophan	0.8
Isoleucine	3.44	Tyrosine	1.42
Leucine	6.5	Valine	4.37

Features

Synergistic Nutrient Blend: Combines the rich amino acid profiles of Casein and Meat peptones to accelerate cell doubling times.
Enhanced Growth Performance: Supports superior biomass yield for demanding or fastidious microorganisms compared to single-source peptones.
Strict Microbial Control: Standard plate count < 5,000 CFU/g; guaranteed negative for Coliforms and Salmonella.
High Quality & Stability: Highly refined with a typical Amino Nitrogen/Total Nitrogen (AN/TN) ratio of 31.25%.

Applications

Preparation of general culture media for academic and industrial microbiology research.
Formulation of specialized nutrient broths and peptone agar plates for robust microbial growth support.
High-yield biomass production of standard bacteria, yeasts, and molds.

How to Use

Weigh the required amount of PolyPeptone powder according to your specific culture media formulation recipe (e.g., standard 2% solution).
Completely dissolve the powder in distilled or deionized water. Ensure adequate agitation; heat gently if necessary.
Adjust the pH to your target range (typically 6.5–7.5; the natural pH of a 2% solution sits around 6.8).
Sterilize the prepared media by autoclaving at 121°C for 15 minutes before inoculation.

Safety

GHS Classification: Not a hazardous substance or mixture according to current regulations. This product contains no components considered to be hazardous to health at given concentrations.

Storage Conditions: Store at room temperature (15°C – 30°C). Keep container tightly closed in a dry, well-ventilated place. Protect from moisture to prevent caking.
Handling: Wear standard laboratory personal protective equipment (safety glasses, lab coat, gloves). Avoid excessive dust formation and breathing vapors or dust.

Documents

INFO

SDS

FAQ

Q1: What is PolyPeptone G258 made of?
A1: Biofargo PolyPeptone is a high-performance enzymatic mixture of Casein Peptone and Meat Peptone, engineered to provide an optimized, balanced nutrient profile for micro-organisms.

Q2: Why use a mixture like PolyPeptone instead of single peptones?
A2: The combination provides a wider variety of peptides and amino acids. Microbial growth assays indicate that some fastidious bacteria show significantly better biomass yields when using this blend than when using casein or meat peptone alone.

Q3: How should Biofargo PolyPeptone be stored?
A3: It should be stored at room temperature in its original container, tightly sealed, and completely protected from moisture and high humidity.

Q4: Is PolyPeptone regulated as dangerous goods for transport?
A4: No. According to the Safety Data Sheet (SDS), it is classified as non-hazardous and not dangerous goods under DOT, TDG, IMDG, and IATA regulations, ensuring hassle-free and economical shipping.

Q5: Can this product be used for clinical or human pharmaceutical manufacturing?
A5: No. This product is intended strictly For Laboratory Research Use Only. It is not approved for human or animal diagnostic, therapeutic, or drug use.

PolyPeptone for Culture Media