{"product_id":"pmsf-protease-inhibitor-100mm","title":"PMSF (100 mM)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePMSF (100mM), also known as Phenylmethylsulfonyl Fluoride (PMSF), is a commonly used serine protease inhibitor designed to protect proteins from enzymatic degradation during cell lysis and protein extraction workflows. During sample preparation, endogenous proteases released from disrupted cells or tissues can rapidly degrade target proteins, resulting in reduced protein recovery, altered protein profiles, and inconsistent downstream analysis. Adding PMSF protease inhibitor to lysis buffers helps maintain protein integrity and improves sample quality for molecular biology applications.\u003c\/p\u003e\n\u003cp\u003eThis 100mM PMSF solution is supplied as a concentrated protease inhibitor stock solution and is commonly added to RIPA lysis buffer or other protein extraction buffers before use. PMSF irreversibly inhibits serine proteases, including trypsin, chymotrypsin, and thrombin, helping prevent proteolytic degradation during protein extraction. The recommended working concentration is typically 1mM PMSF, making it suitable for routine preparation of cell lysates and tissue protein extracts.\u003c\/p\u003e\n\u003cp\u003ePMSF (100mM) is widely used in Western blot (WB), SDS-PAGE, immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and other protein analysis workflows where maintaining intact protein samples is essential. It can be used as an individual protease inhibitor or as a component of customized protease inhibitor formulations according to experimental requirements.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePMSF (100mM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0341S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePMSF Protease Inhibitor Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePhenylmethylsulfonyl Fluoride\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Working Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mM final concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein extraction, Western blot, SDS-PAGE, IP, Co-IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEffective serine protease inhibition:\u003c\/strong\u003e PMSF irreversibly inhibits serine proteases and helps protect target proteins during cell lysis and extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReady-to-use 100mM stock solution:\u003c\/strong\u003e Provides a convenient concentrated PMSF solution for preparation of protein extraction buffers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with RIPA buffer workflows:\u003c\/strong\u003e Can be added to RIPA lysis buffer to reduce proteolytic degradation during protein sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSupports protein integrity preservation:\u003c\/strong\u003e Helps maintain intact protein samples for downstream biochemical and molecular biology analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for customized inhibitor formulations:\u003c\/strong\u003e Can be combined with other protease or phosphatase inhibitors according to experimental requirements.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003ePMSF (100mM) is widely used as a protein extraction protease inhibitor in cell biology, molecular biology, and biochemical research workflows where prevention of protein degradation is required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Used during protein extraction to protect target proteins and improve the reliability of SDS-PAGE and Western blot detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRIPA Buffer Protein Extraction:\u003c\/strong\u003e Added to RIPA lysis buffer as a protease inhibitor supplement to minimize degradation of cellular proteins during lysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Preparation:\u003c\/strong\u003e Protects proteins extracted from cultured mammalian cells, bacteria, yeast, and other biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Helps preserve protein profiles during extraction from animal tissues and complex biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Supports preparation of protein lysates with improved protein stability for antibody-based enrichment workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Helps maintain protein complexes during extraction for protein-protein interaction studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Provides improved protein sample protection before electrophoretic separation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification Workflows:\u003c\/strong\u003e Used as an individual protease inhibitor to protect recombinant or native proteins during purification procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003ePMSF (100mM) should be added to protein extraction buffers immediately before use because PMSF activity decreases over time in aqueous solutions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAdd PMSF stock solution to RIPA lysis buffer or other protein extraction buffers to achieve a final concentration of approximately 1mM.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor routine preparation, add PMSF (100mM) at a 1:100 ratio to achieve the recommended working concentration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePerform cell or tissue lysis under cold conditions to minimize protease activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProceed with downstream applications such as Western blot, SDS-PAGE, IP, or Co-IP after clarification of lysates.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0341S\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePMSF (100mM)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is PMSF protease inhibitor used for?\u003c\/summary\u003e\n\u003cp\u003ePMSF protease inhibitor is used to prevent protein degradation during cell lysis and protein extraction. It inhibits serine proteases released during sample preparation and helps maintain protein integrity for applications such as Western blot, SDS-PAGE, IP, and Co-IP.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is PMSF added to RIPA buffer?\u003c\/summary\u003e\n\u003cp\u003ePMSF is commonly added to RIPA lysis buffer because cell disruption releases endogenous proteases that can degrade target proteins. Adding PMSF helps protect extracted proteins and improves the quality of protein lysates for downstream analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended PMSF concentration for protein extraction?\u003c\/summary\u003e\n\u003cp\u003eThe commonly used final concentration of PMSF in protein extraction buffers is approximately 1mM. PMSF (100mM) stock solution is typically diluted 1:100 into RIPA buffer or other compatible lysis buffers.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich proteases are inhibited by PMSF?\u003c\/summary\u003e\n\u003cp\u003ePMSF is an irreversible inhibitor of many serine proteases, including trypsin, chymotrypsin, and thrombin. It is widely used as a serine protease inhibitor during protein extraction workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan PMSF be used for Western blot sample preparation?\u003c\/summary\u003e\n\u003cp\u003eYes. PMSF is frequently used during Western blot sample preparation to reduce proteolytic degradation and improve preservation of target proteins before SDS-PAGE and antibody detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should PMSF solution be stored?\u003c\/summary\u003e\n\u003cp\u003ePMSF (100mM) should be stored at -20 °C. Proper storage and minimizing repeated freeze-thaw cycles help maintain product performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"1ml","offer_id":52776234156213,"sku":"AP0341S","price":15.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_50b3a67a-2048-4afd-ab18-8a0429187eb5.jpg?v=1783488344","url":"https:\/\/biofargo.com\/products\/pmsf-protease-inhibitor-100mm","provider":"Biofargo","version":"1.0","type":"link"}