Key Features

1. Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.

2. Stable at room temperature.

3. Compatible with standard blue LED and UV transilluminators.

4. Use with blue light reduces mutagenesis for gel excision and subsequent cloning.

Description

Plus Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This single stain gives high sensitivity detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. Plus is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner.

Gel staining with Plus is compatible with downstream applications such as gel extraction and cloning.Plus is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Component

1. Plus Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl

Storage

Store at 2-25°C and protect from light.

Documents

Manual