Description

The kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system is RNase-free, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 ensure that the RNA obtained is free from contamination by protein, DNA, ions, and organic compounds.

Features

Particularly suitable for the purification of RNA from plant samples with low polysaccharides and polyphenols content.
No need to worry about RNA degradation. The whole system is RNase-free.
Effectively remove DNA using DNA-Cleaning Column
Remove DNA without adding DNase
Simple—all operations are completed at room temperature.
Fast—operation can be completed in 30 minutes
Safe—no organic reagent used
High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

Application

It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

Work Flow

Storage

The kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PRL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).

Documents

Plant Total RNA Isolation Kit Instruction Manual

Plant Total RNA Isolation Kit (Low-Polysaccharide/Low-Polyphenol Samples)