Description

Plant Seed Direct PCR Kit I-UNG uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with low polysaccharide and polyphenol content (such as: rice, wheat, tobacco, corn, soybeans, etc.) for use in PCR reactions, so it is particularly suitable Large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for seed samples that still need to be germinated, you can choose to cut out micro-tissue cuts (1-5mg) other than the seed embryo for experiment; for experiments that need to be sampled in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).

The process of releasing genomic DNA from the cleavage reaction system can be completed within 5-10 minutes. There is no need for other processes to remove protein, RNA or secondary metabolites, and the released trace amounts of DNA can be used as a template for PCR reactions.

2× Seed PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can directly use the lysis mixture of plant materials as a template for efficient and specific amplification. This reagent contains the company's D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially modified for direct PCR. Used in conjunction with the lysis reaction system can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.

2× Seed PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× Seed PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the differences in plant samples, the products are divided into two categories: Plant Seed Direct PCR Kit and Plant Seed Direct PCR Plus Kit. In the lysis stage, considering that different types of seeds or tissue materials vary greatly in the amount of reagents used in the lysis process, customers can choose the corresponding plant seed (small and medium) direct PCR kit or plant seed (large) according to the size of their experimental materials Direct PCR kit; in the PCR detection stage, customers can also choose ordinary 2× Seed PCR EasyTM Mix or 2× Seed PCR EasyTM Mix (UNG) which can prevent the contamination of PCR amplification products according to the experimental needs.

Features

No need time-consuming and expensive DNA purification is required.
The sample demand is small, just take a single seed.
No special treatments such as grinding and crushing are required, and the operation is simple.
The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.
Anti-pollution PCR system 2× Seed PCR EasyTM Mix (UNG) can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.

Application

Scope of application: a variety of plant seeds.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenic seeds, plant genotyping, etc.

Work Flow

Electropherogram

Storage

Part I of this kit should be stored at room temperature or 2-8℃.

vReagents Buffer SP1, Buffer SP2, 6× DNA Loading Buffer, under dry conditions, can be stored for 12 months. If you need to store the kit for a longer time, you can store it at 2-8℃.
Part II of this kit should be stored at -20℃.

vReagent 2× Seed PCR Easy Mix, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).