Description

Plant Leaf Direct PCR Plus Kit-UNG adopts a unique lysis buffer system, which can rapidly release genomic DNA from leaf samples of plants with high polysaccharide and polyphenol content (such as cotton, banana, etc.) for PCR reaction. The leaves do not need to be ground or shredded when treated with lysis buffer, making them ideal for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer can be completed within 5-10 minutes, without the need for other processes of deproteinization, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction.

2× Leaf PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can directly use the cleavage product of plant material as a template for efficient and specific amplification. The reagent contains D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially engineered for direct PCR reactions. It can be used in combination with direct lysate to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.

2× Leaf PCR EasyTM Mix (UNG) replaces dTTP with dUTP on the basis of 2× Leaf PCR Mix, and at the same time adds UNG enzyme (racil-N-glycosylase) that can degrade the template containing dUTP. Before the PCR reaction, the PCR product containing uracil is degraded by UNG enzyme. UNG enzyme will not have any effect on the template that does not contain uracil, so as to ensure the specificity and accuracy of amplification and prevent the possibility of large-scale genetic testing. PCR product contamination problems arise.

D-Taq DNA polymerase is a DNA polymerase specially developed for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min. It is especially suitable for Direct PCR reaction.

According to the differences of plant samples, the products are divided into two categories: Plant Leaf Direct PCR Kit and Plant Leaf Direct PCR Plus Kit for polysaccharides and polyphenols; in the PCR detection stage, it can be According to the experimental needs, choose the common 2× Leaf PCR EasyTM Mix or the 2× Leaf PCR EasyTM Mix (UNG) which has the effect of preventing the contamination of PCR amplification products.

Features

No time-consuming and expensive DNA purification
Less material
Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
2X Mix—reducing sample loading errors and reaction system preparation time
Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes
High throughput—lysis reaction can be completed in 96-well PCR plate

Work Flow

Electropherogram

Storage

Part I of this kit should be stored at room temperature or 2-8℃.

Reagents Buffer P1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.

Part II of this kit should be stored at -20℃.

Reagents Buffer 2×Leaf PCR EasyTM Mix, if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).

Documents

Plant Leaf Direct PCR Plus Kit - UNG

Plant Leaf Direct PCR Plus Kit-UNG (Polysaccharide polyphenol plant)