Synonym(s): OmniPur® PIPES, Sodium Salt

Description

Our high-purity PIPES Sodium Salt (Sesquisodium Salt) is supplied as white crystalline powder, with a molecular weight of 335.3 g/mol and CAS no. 100037-69-2. This biochemical-grade reagent is manufactured to meet stringent quality requirements: purity ≥ 99.0% (or ≥ 96.5%, depending on batch), heavy metals ≤ 5 ppm, moisture ≤ 4.0%. In aqueous solution (1.0% at 25 °C), the pH falls between 6.5–7.1. Additionally, the low UV absorbance at 260 nm and 280 nm (≤ 0.05) ensures minimal interference with nucleic acid and protein assays.

PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is widely regarded for its excellent buffering capacity near physiological pH, making it a preferred choice for biological and biochemical experiments requiring stable pH and high purity.

Specifications

Item	Value
Chemical Name	Piperazine, N,N'-Bis(2-Ethanesulfonic Acid), Sesquisodium Salt
Appearance	White Crystal
CAS No.	100037-69-2
Molecular Formula	C8H16.5N2O6S2Na1.5
Molecular Weight	335.3
Grade	High Purity Grade
Purity	≥96.5%
Water Solubility	Colorless, Clear, Transparent
Heavy Metals	≤5 ppm
Moisture	≤4.0%
pH (1.0% in Water at 25℃)	6.5–7.1
UV Absorbance at 260nm	≤0.05
UV Absorbance at 280nm	≤0.05

Features

High purity: ≥ 99.0% grade ensures minimal impurities for sensitive biological applications.
Low heavy metal content: ≤ 5 ppm — reduces risk of metal-induced toxicity or interference in cell culture or enzyme reactions.
Low moisture & stable quality: moisture ≤ 4.0% ensures consistent batch performance and long shelf life.
Low UV absorbance: ≤ 0.05 at 260 nm and 280 nm — minimizes background absorption, ideal for nucleic acid, protein or other spectrophotometric assays.
Optimal buffering capacity at physiological pH: pH 6.5–7.1 in 1.0% aqueous solution — stable buffering near neutral pH range.
Clear, colorless solution when dissolved: ensures visual clarity and ease of use in buffer preparations.

Applications

Preparation of buffers for cell culture, especially when precise pH control is required.
Use in molecular biology protocols, such as DNA/RNA purification, enzyme reactions, electrophoresis, where low UV background is critical.
Formulation of biochemical assay buffers — enzymatic assays, protein work, immunoassays, where purity and minimal metal contamination matter.
Physiological-pH buffer systems for in-vitro biological research.
Any application demanding high-quality, low-contaminant buffering reagents.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

COA

SDS

PIPES Sodium Salt 99.0% High Purity Grade