Synonym(s): 1,4-Piperazinediethanesulfonic acid sodium salt, Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt

Description

PIPES-Na (Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt) is a high-quality biochemical-grade buffer reagent supplied as a white powder. With a molecular weight of 324.3 and ≥ 99% purity, our PIPES-Na is designed to meet rigorous research standards. Upon dissolution in water (1% w/v), it forms a clear, colorless solution with minimal absorbance at 260 nm and 280 nm — reflecting its high purity and minimal contaminating nucleic acids or proteins. The buffer exhibits a working pH of 4.0–5.0 (1% solution at 25 °C), making it a reliable pH buffer for biochemical and cell biology applications.

Specifications

Item	Value
Chemical Name	PIPES Sodium Salt
Appearance	White Powder
CAS No.	10010-67-0
Molecular Formula	C8H17N2NaO6S2
Molecular Weight	324.3
Grade	Biochemical Grade
Purity	≥99%
Moisture	≤3.0%
Infrared	Complies with Specifications
pH (1% Di H2O, 25℃)	4.0–5.0
Solubility (1.0% Aqueous Solution)	Clear Colorless Solution
A280 (1.0% Aqueous Solution)	≤0.040
A260 (1.0% Aqueous Solution)	≤0.050

Features

High purity (≥ 99%) — ensures minimal background impurities for sensitive experiments.
Biochemical grade quality — suitable for molecular biology, cell culture, and protein/RNA work.
Clear, colorless solution — dissolves readily to produce a transparent 1% aqueous solution, facilitating accurate downstream analysis.
Low nucleic acid and protein absorbance — A260 ≤ 0.050, A280 ≤ 0.040 for 1% solution, avoiding interference in spectrophotometric assays.
Consistent pH stability — pH 4.0–5.0 (1% solution at 25 °C), providing a stable acidic buffer environment.
Low moisture content — ≤ 3.0% moisture helps maintain stability and long shelf-life.

Applications

Molecular biology & nucleic acid research — ideal for RNA/DNA extraction, purification, and enzymatic reactions requiring low background absorbance.
Protein biochemistry — useful in protein purification, enzyme assays, or chromatography workflows where pH stability and buffer purity are critical.
Cell culture & tissue work — suitable for preparing buffer solutions in various cell biology experiments, particularly when an acidic buffer environment is required.
Buffer preparation for sensitive assays — recommended for downstream applications such as spectrophotometry, fluorometry, or any assay needing minimal background interference.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

MSDS

PIPES-Na 99% Biochemical Grade