{"product_id":"phosphatase-inhibitor-cocktail-100x","title":"Phosphatase Inhibitor Cocktail (100X)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePhosphatase Inhibitor Cocktail (100X) is a broad-spectrum phosphatase inhibitor mix designed to preserve protein phosphorylation status during cell lysis and protein extraction workflows. During sample preparation, endogenous phosphatases released from disrupted cells or tissues can rapidly remove phosphate groups from target proteins, resulting in reduced phosphoprotein signals and inaccurate analysis of phosphorylation-dependent signaling pathways. This 100X phosphatase inhibitor cocktail helps prevent protein dephosphorylation and maintains native phosphorylation states for downstream applications.\u003c\/p\u003e\n\u003cp\u003eFormulated with commonly used phosphatase inhibitors including sodium fluoride, sodium pyrophosphate, β-glycerophosphate, and sodium orthovanadate, this protein phosphatase inhibitor cocktail provides broad-spectrum inhibition against endogenous serine\/threonine phosphatases and protein tyrosine phosphatases. It is suitable for protecting phosphorylated proteins during preparation of cell lysates, tissue extracts, and other biological samples.\u003c\/p\u003e\n\u003cp\u003ePhosphatase Inhibitor Cocktail (100X) is supplied as a convenient concentrated solution and is typically added to lysis buffers at a 1:100 dilution before sample extraction. Combined with protease inhibitors, it provides enhanced protection of protein samples for Western blot, phospho-Western blot, immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and phosphorylation signaling pathway analysis.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePhosphatase Inhibitor Cocktail (100X)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0431\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePhosphatase Inhibitor Cocktail\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eWorking Dilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1:100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTarget Enzymes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSerine\/Threonine Phosphatases, Tyrosine Phosphatases, Acid Phosphatases, Alkaline Phosphatases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein Extraction, Western Blot, Phospho-Western Blot, Immunoprecipitation, Co-IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCells, tissues, bacteria, fungi, plants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad-spectrum phosphatase inhibition:\u003c\/strong\u003e Effectively inhibits multiple endogenous phosphatases including serine\/threonine and protein tyrosine phosphatases.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePreserves protein phosphorylation:\u003c\/strong\u003e Helps maintain phosphorylation states during cell lysis and protein extraction for accurate phosphoprotein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient 100X concentrated formulation:\u003c\/strong\u003e Easy addition to RIPA buffer, NP-40 lysis buffer, and other protein extraction reagents at a 1:100 dilution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSupports phospho-signaling research:\u003c\/strong\u003e Suitable for studies involving MAPK, PI3K\/Akt, JAK\/STAT, and other phosphorylation-dependent pathways.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with common protein workflows:\u003c\/strong\u003e Designed for Western blot, phospho-Western blot, immunoprecipitation, and protein interaction studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003ePhosphatase Inhibitor Cocktail (100X) is widely used as a protein extraction additive to protect phosphorylation-dependent protein modifications in molecular biology, cell biology, and biochemical research workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePhospho-Western Blot Analysis:\u003c\/strong\u003e Preserves phosphorylated proteins during sample preparation to improve detection of phosphorylation-specific targets such as phospho-AKT, phospho-ERK, phospho-mTOR, and phospho-STAT proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Protein Extraction:\u003c\/strong\u003e Added to cell lysis buffers to inhibit endogenous phosphatase activity after cell disruption and maintain protein phosphorylation status.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Supports preparation of tissue lysates where endogenous phosphatases may affect phosphorylation analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRIPA Lysis Buffer Supplement:\u003c\/strong\u003e Commonly combined with RIPA buffer and protease inhibitors for whole-cell protein extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Helps preserve phosphorylated epitopes during antibody-based protein enrichment workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Supports studies of phosphorylation-regulated protein interactions and signaling complexes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSignal Transduction Research:\u003c\/strong\u003e Suitable for analysis of cellular signaling pathways regulated by reversible phosphorylation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Sample Preparation:\u003c\/strong\u003e Used for preparing stable protein lysates for biochemical analysis and downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal phosphoprotein preservation, Phosphatase Inhibitor Cocktail (100X) should be added to freshly prepared lysis buffer immediately before sample processing.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd phosphatase inhibitor cocktail to lysis buffer at a 1:100 ratio to obtain the recommended working concentration.\u003c\/li\u003e\n\u003cli\u003eFor example, add 10 μL of 100X phosphatase inhibitor cocktail to 1 mL of lysis buffer.\u003c\/li\u003e\n\u003cli\u003ePrepare inhibitor-containing lysis buffer immediately before use and avoid long-term storage after dilution.\u003c\/li\u003e\n\u003cli\u003eCombine with protease inhibitor cocktail when protection against both protein degradation and dephosphorylation is required.\u003c\/li\u003e\n\u003cli\u003ePerform cell or tissue lysis under cold conditions to minimize endogenous enzyme activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0431\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePhosphatase Inhibitor Cocktail (100X)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Phosphatase Inhibitor Cocktail (100X) used for?\u003c\/summary\u003e\n\u003cp\u003ePhosphatase Inhibitor Cocktail (100X) is used to inhibit endogenous phosphatases during protein extraction and cell lysis. It helps preserve protein phosphorylation states for Western blot, phospho-Western blot, immunoprecipitation, and signaling pathway analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy should phosphatase inhibitors be added to lysis buffer?\u003c\/summary\u003e\n\u003cp\u003eCell lysis releases endogenous phosphatases that can remove phosphate groups from proteins and reduce phosphoprotein signals. Adding a phosphatase inhibitor cocktail helps prevent dephosphorylation and maintains the original phosphorylation status of target proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow do you use a 100X phosphatase inhibitor cocktail?\u003c\/summary\u003e\n\u003cp\u003eThe recommended dilution is 1:100. For example, add 10 μL of Phosphatase Inhibitor Cocktail (100X) to 1 mL of lysis buffer immediately before protein extraction.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich phosphatases are inhibited by this cocktail?\u003c\/summary\u003e\n\u003cp\u003eThis phosphatase inhibitor mix provides broad-spectrum inhibition against acid phosphatases, alkaline phosphatases, serine\/threonine phosphatases, and protein tyrosine phosphatases.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this phosphatase inhibitor cocktail be used with RIPA buffer?\u003c\/summary\u003e\n\u003cp\u003eYes. Phosphatase Inhibitor Cocktail (100X) can be added to RIPA lysis buffer before protein extraction. It is commonly used together with protease inhibitors for complete protection of protein samples.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between protease inhibitor and phosphatase inhibitor?\u003c\/summary\u003e\n\u003cp\u003eProtease inhibitors prevent protein degradation caused by proteases, while phosphatase inhibitors prevent removal of phosphate groups from proteins. For phosphoprotein analysis, both inhibitors are often used together during cell lysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich downstream applications are compatible with this product?\u003c\/summary\u003e\n\u003cp\u003eThis product is suitable for Western blot, phospho-Western blot, SDS-PAGE sample preparation, immunoprecipitation, Co-IP, and phosphorylation signaling research workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"1mL","offer_id":52776289927349,"sku":"AP0431","price":63.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6_0fb9db49-3779-4885-a7fc-4c534fdcb2e5.jpg?v=1783494433","url":"https:\/\/biofargo.com\/products\/phosphatase-inhibitor-cocktail-100x","provider":"Biofargo","version":"1.0","type":"link"}