Specification

Package	1 KU/5 X1 KU/250 U
Full name	Phi29 DNA Polymerase
Applications	Routine PCR ，High throughput PCR， Multiplex PCR， Genotyping，Rolling circle amplification (RCA)
Summary	This product is derived from recombinant E. coli carrying Bacillus subtilis phage phi29 clone gene 2, which was expressed by E. coli and isolated and purified multiple times. Phi29 is a DNA polymerase with high sequential synthesis capacity and strand displacement for efficient isothermal DNA amplification with 3'-5' exonuclease (proofreading) activity.
Features	1. High-precision DNA synthesis 2. Extremely high amplified DNA yield can be obtained even from a very small amount of template 3. The amplification products can be directly used in downstream applications (PCR, restriction enzyme digestion, SNP genotyping, etc.).
Composition	Phi29 DNA Polymerase (10U/ul), 10 x Phi29 Buffer.
Reaction Buffer	10X phi29 Buffer
Unit Definition	1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 min at 30°C.
Thermal Inactivation	Heat at 60 °C for 10 min to lose activity.
Concentration	10 U/μl
Storage Buffer	50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
Component	Phi29 DNA Polymerase (10U/ul), 10 x Phi29 Buffer.

Storage

-20°C