Description

This product is derived from recombinant E. coli carrying Bacillus subtilis phage phi29 clone gene 2, which was expressed by E. coli and isolated and purified multiple times. Phi29 is a DNA polymerase with high sequential synthesis capacity and strand displacement for efficient isothermal DNA amplification with 3'–5' exonuclease (proofreading) activity.

The catalog number has been updated from P310141-0001 to P310141-1KU.

Specification

Full name	Phi29 DNA Polymerase
Composition	Phi29 DNA Polymerase (10 U/μL), 10 × Phi29 Buffer.
Reaction Buffer	10X phi29 Buffer
Unit Definition	1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 min at 30°C.
Thermal Inactivation	Heat at 60°C for 10 min to lose activity.
Concentration	10 U/μL
Storage Buffer	50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
Component	Phi29 DNA Polymerase (10 U/μL), 10 × Phi29 Buffer.

Features

High-precision DNA synthesis
Extremely high amplified DNA yield can be obtained even from a very small amount of template
The amplification products can be directly used in downstream applications (PCR, restriction enzyme digestion, SNP genotyping, etc.).

Applications

Routine PCR
High throughput PCR
Multiplex PCR
Genotyping
Rolling circle amplification (RCA)

Storage

-20°C

Phi29 DNA Polymerase