Description

This kit uses the spin column and formula developed by our company, which can efficiently recover high-purity DNA fragments from the PCR system. The purification column can efficiently bind DNA, and is matched with a unique formula reagent to directly remove the primer dimer without gel recovery.

The kit recovers a wide range of DNA fragments. Under normal conditions, DNA fragments as short as 60bp can be recovered. A minimum of 30μl of eluent can be used to increase the concentration of recovered DNA.

The kit is easy to operate and has few steps. It only needs several centrifugation to process multiple samples at the same time, and high-purity recovered DNA fragments can be obtained in 15 minutes.

Features

Wide range of DNA recovery: DNA fragments as short as 60bp and as large as 10kb can be recovered.
High recovery efficiency: The recovery efficiency is generally more than 80%, and the highest can reach more than 95%.
Fast speed: Easy to operate, DNA fragment recovery can be completed within 15 minutes and primer dimer can be directly removed without gel recovery.
Safety: No organic reagent extraction is required.
High quality: The recovered DNA fragments are of high purity, which can meet various subsequent experiments.

Application

This kit is suitable for recovering DNA fragments (60bp-10kb) in PCR products.

Appliaction of recovering DNA fragments:

The DNA recovered by Gel Extraction Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, ligation, PCR, sequencing and other experiments.

Components

Buffer BD*
Buffer BD-S
Buffer WB1
Buffer EB
DNA-Only Column
Instruction

Note: Buffer BD contains irritating chaotropic salt, please wear gloves and take relevant protective measures during operation.

Work Flow

Storage

This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

Documents

PCR Purification Kit Instruction Manual-V1.0-EN