Descprition

This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Taq Hero is a new generation of DNA polymerase modified by Foregene based on Taq enzyme.

Specifications:

Higher fidelity: 6 times that of ordinary Taq enzyme;
Faster amplification speed: the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;
More template adaptability: it can efficiently amplify various complex DNA templates with high GC content and difficult to amplify;
Higher Amplification efficiency: the number of amplification cycles is lower than that of ordinary Taq enzyme;
Stronger Environmental tolerance: placed at 37°C for a week, maintaining more than 90% activity;
It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity.

Product components

2× PCR HeroTM Mix include:0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8),100mM KCl, 5mM MgCl2,other stabilizers and enhancers.

Kit Series

PH-01013:2× PCR HeroTM Mix 1.7ml ×3
PH-01014:2× PCR HeroTM Mix 1.7ml ×6
PH-01015:2× PCR HeroTM Mix 1.7ml ×24

Features

The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Application

PCR amplification of DNA fragments
DNA labeling
DNA sequencing
PCR plus A tail

Practical examples

1.Reaction System

2× PCR HeroTM Mix	10μl
Template DNA	xμl
Primer 1(10μM)	0.4μl
Primer 2(10μM)	0.4μl
ddH2O	Fill to 20μl
Total volume	20µl

2.Reaction conditions

Temperature	Time	Cycles
94°C	3min	1
94°C	10sec	25-40
55-65°C	10sec
72°C	≥10sec (5-20sec/kb)^1*
72°C	5min	1

1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.

Storage and Shelf life

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

Documents

PCR Hero™ Instruction Manual-V1.0

PCR Hero ™