{"product_id":"onepot-pro-dna-library-prep-kit-v4-enzymatic-12972es","title":"OnePot Pro DNA Library Prep Kit V4 (Enzymatic)-12972ES","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eOnePot Pro DNA Library Prep Kit V4 is is an advanced enzymatic DNA library preparation kit designed for compatibility with both Illumina™ and MGI™ next-generation sequencing(NGS) platforms. By utilizing high-efficiency enzymatic fragmentation, this kit eliminates the need for traditional mechanical shearing methods such as sonication, streamlining the workflow and minimizing hands-on time.\u003c\/p\u003e\n\u003cp\u003eThe kit integrates DNA fragmentation and end-repair into a single-step reaction, significantly reducing overall library preparation time and cost. It is suitable for a broad range of sample types, including plant genomes, animal genomes, microbial genomes, and more, with a flexible DNA input range from 1 ng to 1 μg.\u003c\/p\u003e\n\u003cp\u003eThe optimized enzymatic fragmentation ensures consistent and uniform library fragment sizes across different species, minimizing variation and enhancing sequencing performance. The prepared libraries are fully compatible with Illumina™ or MGI™ adapters and primers, enabling seamless downstream sequencing on respective platforms.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBroad Sample Compatibility: Supports a wide range of input DNA from 100 pg to 1 µg, covering all common sample types.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh Fragment Recovery: Post-fragmentation recovery rate exceeds 60%.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eExcellent Library Uniformity: Consistent library distribution across different species under the same preparation conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStrong Inhibitor Tolerance: High success rate of direct library preparation from complex samples containing inhibitors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAutomation-Friendly: Single-use reagents remain stable for 4 weeks at 4°C or 25°C, facilitating automation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDNA Library Preparation; \u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWhole Genome Sequencing(WGS); \u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWhole Exome or other Targeted Capture Sequencing; \u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAmplicon Sequencing;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmunoprecipitation Sequencing;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMetagenomic Sequencing; \u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eComponents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eComponents No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e12972ES08\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e12972ES24\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e12972ES96\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e12972-A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSmearase™ Buffer 4.0\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e960 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e12972-B\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSmearase™ Enzyme 4.0\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e960 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e12972-C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLigation Enhancer 4.0\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e720 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e3 × 960 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e12972-D\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRapid DNA Ligase 4.0\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 480 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e12972-E\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2× Ultima HF Amplification Mix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e600 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e3 × 800 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for one year.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-4_1024x1024_b4dcd606-1e5b-4784-b3ad-7b593b42477d.jpg?v=1776390441\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. The workflow of OnePot Pro DNA Library Prep Kit\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eFlexible and High-Efficiency Fragmentation\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-5_1024x1024_9ec47212-914c-4037-b281-f492d00b0ce5.jpg?v=1776390511\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Fragment size distribution of 250 ng human gDNA using the 12972 fragmentation module.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eExcellent Buffer Tolerance\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-6_1024x1024_f280d00b-a42f-4564-8e56-6bbf42f3f867.jpg?v=1776392459\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 3. Fragmentation performance of 12972ES in different reaction buffers(H2O (pH 7.0), 1×TE (pH 7.5–8.0), 1×AE (pH 8.3), and 1×EB (pH 8.5)).\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFragmentation reactions were performed using 12972ES with 500 ng of 293 gDNA at 30°C for 20 minutes in four different buffers. When 10 μL of each buffer was used with DEPC-treated water, the fragment sizes were similar to those in pure DEPC water. Using AE or EB as the full reaction buffer resulted in slightly larger fragments.\u003c\/p\u003e\n\u003ch2\u003eBroad Species Compatibility\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972_1024x1024_06db093a-e71b-406d-a9bd-8c92a3d12d64.jpg?v=1776392459\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 4. Library uniformity testing across different species using 12972 under identical fragmentation conditions.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDifferent DNA samples were fragmented with 12972 under identical conditions. The libraries showed consistent sizes (~450 bp \/ ~320 bp) with good uniformity across species.\u003c\/p\u003e\n\u003ch2\u003eExcellent Sequencing Data Performance\u003c\/h2\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-8_1024x1024_646ea5b1-f222-4e48-b230-d51b239460bb.jpg?v=1776392459\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 5. Performance of 10X WGS Data on Human and Bovine Samples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e12972 demonstrates higher gene coverage compared to Supplier W*. Both false chimeric rate and hairpin artifacts are lower or comparable to those of Supplier W*.\u003c\/p\u003e\n\u003ch2\u003eStrong Stability\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-9_1024x1024_485747a5-676b-479f-bfc7-fa8912df962a.jpg?v=1776392459\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 6. Library yield stability after storage at 4°C and 25°C for 4 weeks. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eNo decrease in library yield was observed after 4 weeks of storage at 4°C and 25°C, highlighting the kit’s suitability for automation.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch3\u003eSafety Data Sheet\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972-MSDS-HB250620_1.pdf?v=1776395193\"\u003e12972_MSDS_HB250620_EN.PDF\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eManuals\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12972ES-Manual_-Ver.EN20241225.pdf?v=1776395192\"\u003e12972_Manual_HB20260401.PDF\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Yeasenbio","offers":[{"title":"8T","offer_id":52439216062645,"sku":"12972ES08","price":129.0,"currency_code":"USD","in_stock":true},{"title":"24T","offer_id":52439216095413,"sku":"12972ES24","price":461.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":52439216128181,"sku":"12972ES96","price":1772.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5a8276a7-faff-49c0-8780-8c4b8a161378.jpg?v=1776393038","url":"https:\/\/biofargo.com\/products\/onepot-pro-dna-library-prep-kit-v4-enzymatic-12972es","provider":"Biofargo","version":"1.0","type":"link"}