{"product_id":"np-40-lysis-buffer","title":"NP-40 Lysis Buffer","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eNP-40 Lysis Buffer is a mild non-denaturing cell lysis buffer designed for efficient protein extraction while maintaining native protein structure and protein-protein interactions. During protein sample preparation, overly harsh extraction conditions may disrupt protein complexes or affect antibody-based enrichment workflows, while insufficient lysis can reduce protein recovery. This NP-40 cell lysis buffer provides a balanced extraction environment to help researchers prepare reliable cell and tissue lysates for Western blot, SDS-PAGE, immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and ELISA applications.\u003c\/p\u003e\n\u003cp\u003eFormulated with a nonionic detergent system containing NP-40, this protein extraction buffer enables gentle disruption of cellular membranes while supporting non-denaturing protein extraction. Compared with stronger RIPA lysis buffers, NP-40 Lysis Buffer is particularly suitable for experiments requiring preservation of protein complexes, native protein interactions, and cytoplasmic protein analysis.\u003c\/p\u003e\n\u003cp\u003eNP-40 Lysis Buffer is compatible with a wide range of biological samples, including animal cells, tissues, plant samples, bacteria, and yeast. It is part of a protein extraction reagent portfolio designed to provide researchers with flexible lysis solutions according to sample type, protein characteristics, and downstream experimental requirements.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNP-40 Lysis Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0421\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMild NP-40 Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eBuffer Composition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, EDTA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetergent Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNonionic Detergent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein extraction, Western Blot, SDS-PAGE, Immunoprecipitation, Co-IP, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCells, tissues, plants, bacteria, yeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMild non-denaturing protein extraction:\u003c\/strong\u003e Provides gentle cell lysis conditions to help maintain native protein structure and protein-protein interactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNP-40 based cell lysis system:\u003c\/strong\u003e Uses a nonionic detergent formulation for efficient membrane disruption with reduced impact on protein complexes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Suitable for protein extraction from animal cells, tissues, plant samples, bacteria, and yeast.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized for antibody-based workflows:\u003c\/strong\u003e Compatible with immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and protein interaction studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReady-to-use protein extraction reagent:\u003c\/strong\u003e Simplifies preparation of whole-cell lysates and tissue protein extracts for downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eNP-40 Lysis Buffer is widely used as a mild protein extraction reagent in molecular biology, cell biology, and biochemical research workflows requiring efficient lysis with preservation of protein interactions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Preparation of cell lysates and tissue extracts for SDS-PAGE separation and Western blot detection of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Provides a suitable lysis environment for antibody-based protein enrichment while maintaining native protein conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Supports protein-protein interaction studies by helping preserve protein complexes during extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNative Protein Extraction:\u003c\/strong\u003e Suitable for experiments requiring non-denaturing protein extraction and functional protein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Protein Extraction:\u003c\/strong\u003e Used for preparation of whole-cell lysates from adherent and suspension cultured cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Supports extraction of proteins from animal and plant tissue samples after mechanical disruption.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eELISA Sample Preparation:\u003c\/strong\u003e Provides protein lysates suitable for antibody-based detection workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Interaction Research:\u003c\/strong\u003e Used in biochemical studies involving protein complexes, signaling pathways, and cellular regulation mechanisms.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBacterial and Yeast Protein Extraction:\u003c\/strong\u003e Can be combined with mechanical disruption or enzymatic treatment for microbial protein preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal protein extraction performance, sample processing should be performed under cold conditions to minimize protein degradation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd appropriate protease inhibitors or phosphatase inhibitors before use according to experimental requirements.\u003c\/li\u003e\n\u003cli\u003eLyse samples on ice or at 4 °C to maintain protein stability.\u003c\/li\u003e\n\u003cli\u003eIncubate cells or tissue samples with NP-40 Lysis Buffer until sufficient disruption is achieved.\u003c\/li\u003e\n\u003cli\u003eCentrifuge lysates at 10,000–14,000 ×g for 3–5 minutes and collect the clarified supernatant for downstream applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause NP-40 Lysis Buffer contains detergent components, Bradford protein assays may produce inaccurate results. Detergent-compatible methods such as BCA protein assays are recommended for protein concentration measurement.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0421\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNP-40 Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is NP-40 Lysis Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eNP-40 Lysis Buffer is a mild cell lysis and protein extraction buffer used to prepare protein lysates from cells and tissues for Western blot, SDS-PAGE, immunoprecipitation, Co-IP, and ELISA applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between NP-40 Lysis Buffer and RIPA Lysis Buffer?\u003c\/summary\u003e\n\u003cp\u003eNP-40 Lysis Buffer provides mild non-denaturing extraction conditions that are suitable for preserving protein interactions. RIPA buffers generally provide stronger lysis capability and are commonly selected when higher protein solubilization is required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan NP-40 Lysis Buffer be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. NP-40 Lysis Buffer is commonly used to prepare protein samples for SDS-PAGE and Western blot analysis. The extracted lysates can be analyzed using standard antibody detection workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs NP-40 Lysis Buffer suitable for immunoprecipitation and Co-IP?\u003c\/summary\u003e\n\u003cp\u003eYes. Because NP-40 provides mild nonionic detergent-based lysis, it is frequently used for immunoprecipitation and co-immunoprecipitation workflows where maintaining protein complexes is important.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of samples can be extracted with NP-40 Lysis Buffer?\u003c\/summary\u003e\n\u003cp\u003eNP-40 Lysis Buffer can be used for animal cells, tissues, plant samples, bacteria, and yeast. Optimization of lysis conditions may be required depending on sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Bradford assay be used after NP-40 extraction?\u003c\/summary\u003e\n\u003cp\u003eNo. NP-40 is a detergent and may interfere with Bradford protein quantification. Detergent-compatible methods such as BCA assays are recommended for protein concentration measurement.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes NP-40 Lysis Buffer preserve protein-protein interactions?\u003c\/summary\u003e\n\u003cp\u003eYes. Compared with stronger detergent-based lysis buffers, NP-40 Lysis Buffer provides gentler extraction conditions that are more suitable for preserving native protein complexes and protein interactions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"100mL","offer_id":52776255226037,"sku":"AP0421","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_3dbc9b60-4605-41c9-aab4-ab5e1b2a4481.jpg?v=1783488655","url":"https:\/\/biofargo.com\/products\/np-40-lysis-buffer","provider":"Biofargo","version":"1.0","type":"link"}