{"product_id":"nkcryo-serum-free-nk-cell-cryopreservation-solution","title":"NKCryo - Serum-Free NK Cell Cryopreservation Solution","description":"\u003c!--\nSEO TITLE:\nNK Cell Cryopreservation Medium | Serum-Free NK Freezing\n\nMETA DESCRIPTION:\nBiofargo NKCryo helps laboratories reduce NK cell loss during freezing, transport and thawing with a serum-free, protein-free cryopreservation medium.\n\nRECOMMENDED URL:\n\/products\/nk-cell-cryopreservation-medium\n\nPRIMARY KEYWORDS:\nNK Cell Cryopreservation Medium\nNK Cell Freezing Medium\nSerum-Free NK Cell Cryopreservation Medium\nHuman NK Cell Cryopreservation Medium\nNatural Killer Cell Cryopreservation\n\nSECONDARY KEYWORDS:\nSerum-Free NK Cell Freezing Medium\nNK Cell Preservation Medium\nImmune Cell Cryopreservation Medium\nAnimal Component-Free Cryopreservation Medium\nProtein-Free Cryopreservation Medium\nHuman NK Cell Freezing\nPBMC-Derived NK Cell Cryopreservation\nCord Blood NK Cell Cryopreservation\nHigh-Density NK Cell Freezing\nPost-Thaw NK Cell Viability\nNK Cell Recovery After Thawing\nLong-Term NK Cell Storage\n10% DMSO Cryopreservation Medium\n--\u003e\n\n\u003cp\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"Biofargo NKCryo Serum-Free NK Cell Cryopreservation Medium\",\n  \"description\": \"Serum-free, animal component-free and protein-free cryopreservation medium designed to protect human NK cells during freezing, dry ice transportation, thawing and long-term storage.\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Biofargo\"\n  },\n  \"category\": \"Cell Cryopreservation Medium\",\n  \"sku\": \"XRNKCRYO-0-100\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Cell Type\",\n      \"value\": \"Human NK cells\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Application\",\n      \"value\": \"Human NK cell cryopreservation and post-thaw recovery\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Format\",\n      \"value\": \"Serum-free, animal component-free and protein-free\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"DMSO Concentration\",\n      \"value\": \"10% (v\/v)\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Supported Freezing Density\",\n      \"value\": \"1 × 10^6 to 5 × 10^8 cells\/mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage\",\n      \"value\": \"2–8°C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Shelf Life\",\n      \"value\": \"12 months\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Research Use\",\n      \"value\": \"For research use only\"\n    }\n  ]\n}\n\u003c\/script\u003e\n\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is Biofargo NKCryo used for?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Biofargo NKCryo is a serum-free NK cell cryopreservation medium designed to protect human natural killer cells during freezing, dry ice transportation, thawing and long-term storage.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is Biofargo NKCryo a serum-free NK cell freezing medium?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. Biofargo NKCryo contains no serum, no human- or animal-derived ingredients, and no protein components.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the DMSO concentration in Biofargo NKCryo?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Biofargo NKCryo contains 10% DMSO by volume.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What NK cell freezing density does Biofargo NKCryo support?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Biofargo NKCryo supports human NK cell freezing densities ranging from 1 × 10^6 to 5 × 10^8 cells per milliliter.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Can Biofargo NKCryo be used for PBMC-derived and cord blood-derived NK cells?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Biofargo NKCryo is intended for human NK cells, including NK cells generated from peripheral blood or cord blood mononuclear cell sources.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How should NK cells frozen in Biofargo NKCryo be stored?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Cells may be frozen overnight at -80°C using a controlled-rate freezing container and then transferred to liquid nitrogen at approximately -196°C for long-term storage.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How should NK cells be thawed after cryopreservation?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Thaw the cells in a 37°C water bath for approximately 2 to 3 minutes, dilute with at least three volumes of serum-free NK cell culture medium or physiological saline, and centrifuge to remove the cryopreservation medium.\"\n      }\n    }\n  ]\n}\n\u003c\/script\u003e\n\u003c\/p\u003e\n\n\u003cstyle\u003e\n.pdp-container {\n  font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,Helvetica,Arial,sans-serif;\n  color:#333333;\n  line-height:1.6;\n  max-width:1200px;\n  margin:0 auto;\n  padding:15px;\n}\n\n.pdp-container h2 {\n  font-size:24px;\n  color:#0B3C5D;\n  border-bottom:2px solid #328CC1;\n  padding-bottom:8px;\n  margin-top:40px;\n  margin-bottom:20px;\n  font-weight:600;\n}\n\n.pdp-container h3 {\n  font-size:18px;\n  color:#1D2731;\n  margin-top:30px;\n  margin-bottom:15px;\n  font-weight:600;\n}\n\n.pdp-container p {\n  margin-bottom:16px;\n  font-size:15px;\n  color:#4A4A4A;\n  text-align:justify;\n}\n\n.pdp-container ul {\n  padding-left:20px;\n  margin-bottom:24px;\n}\n\n.pdp-container li {\n  margin-bottom:10px;\n  font-size:15px;\n  color:#4A4A4A;\n  list-style-type:square;\n}\n\n.pdp-table-responsive {\n  width:100%;\n  overflow-x:auto;\n  margin:25px 0;\n  border:1px solid #E0E0E0;\n  border-radius:6px;\n}\n\n.pdp-table {\n  width:100%;\n  border-collapse:collapse;\n  min-width:600px;\n  background:#FFFFFF;\n}\n\n.pdp-table td {\n  padding:12px 16px;\n  border-bottom:1px solid #E0E0E0;\n  font-size:14px;\n}\n\n.pdp-table tr:nth-child(even) {\n  background:#F8F9FA;\n}\n\n.pdp-table td strong {\n  color:#0B3C5D;\n}\n\n.pdp-img-container {\n  max-width:650px;\n  margin:24px auto;\n  padding:10px;\n  border:1px solid #EEEEEE;\n  border-radius:8px;\n  background:#FFFFFF;\n  text-align:center;\n}\n\n.pdp-img-container img {\n  max-width:100%;\n  height:auto;\n}\n\n.pdp-img-placeholder {\n  min-height:260px;\n  display:flex;\n  align-items:center;\n  justify-content:center;\n  padding:30px;\n  border:1px dashed #AAB7C4;\n  border-radius:6px;\n  background:#F8F9FA;\n  color:#687784;\n  font-size:14px;\n  text-align:center;\n}\n\n.pdp-img-caption {\n  margin:10px 0 0;\n  font-size:13px;\n  color:#6B6B6B;\n  text-align:center;\n}\n\n.pdp-container details {\n  background:#F8F9FA;\n  border:1px solid #E0E0E0;\n  border-radius:6px;\n  margin-bottom:12px;\n  padding:14px 18px;\n}\n\n.pdp-container summary {\n  font-weight:600;\n  color:#0B3C5D;\n  cursor:pointer;\n}\n\n.pdp-note {\n  padding:14px 18px;\n  margin:22px 0;\n  background:#F4F8FB;\n  border-radius:0 6px 6px 0;\n}\n\n.pdp-note p {\n  margin:0;\n  color:#3E5668;\n}\n\n@media (max-width:767px) {\n  .pdp-container {\n    padding:10px;\n  }\n\n  .pdp-container h2 {\n    font-size:21px;\n  }\n\n  .pdp-table {\n    min-width:600px;\n  }\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"pdp-container\"\u003e\n\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cp\u003eHuman NK cells can be particularly sensitive to freezing and thawing. Loss of membrane integrity, reduced post-thaw viability and incomplete cell recovery may disrupt downstream expansion, phenotyping and functional assays. These challenges become more significant when laboratories need to freeze highly concentrated NK cell preparations, transport frozen samples on dry ice or establish reproducible long-term cell banks.\u003c\/p\u003e\n\n\u003cp\u003eBiofargo NKCryo is a serum-free NK cell cryopreservation medium developed specifically for human natural killer cell preservation. Its formulation provides cryoprotective components and nutritional support intended to reduce cellular stress during freezing, dry ice transportation and thawing while helping preserve cell viability and recovery after storage.\u003c\/p\u003e\n\n\u003cp\u003eThis human NK cell freezing medium contains no human- or animal-derived ingredients and no protein components, helping reduce the introduction of heterogeneous biological materials into frozen cell preparations. The formulation contains 10% DMSO and uses cell culture-grade, low-endotoxin raw materials.\u003c\/p\u003e\n\n\u003cp\u003eBiofargo NKCryo supports a broad freezing-density range of 1 × 10\u003csup\u003e6\u003c\/sup\u003e to 5 × 10\u003csup\u003e8\u003c\/sup\u003e cells\/mL. It is suitable for human NK cells, including NK cells derived from peripheral blood mononuclear cells and cord blood mononuclear cells, and can be integrated into NK cell expansion, cryopreservation and post-thaw recovery workflows.\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eResearch use only:\u003c\/strong\u003e Biofargo NKCryo is intended for laboratory research applications and is not intended for direct therapeutic or diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBiofargo NKCryo NK Cell Cryopreservation Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRNKCRYO-0-100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum-free human NK cell freezing medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePrimary Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman NK cell cryopreservation, frozen transportation and post-thaw recovery\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplicable Cells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman NK cells, including peripheral blood- and cord blood-derived NK cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum-free, animal component-free and protein-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDMSO Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10% (v\/v)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupported Freezing Density\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e to 5 × 10\u003csup\u003e8\u003c\/sup\u003e cells\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤0.1 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacterial Testing\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungal Testing\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCell Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-80°C for short-term storage; liquid nitrogen at approximately -196°C for long-term storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNK Cell-Specific Cryopreservation Solution\u003c\/strong\u003e — Biofargo NKCryo is designed specifically for human natural killer cell cryopreservation workflows, helping laboratories maintain NK cell quality during freezing, transportation and thawing procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSerum-Free and Animal Component-Free Formulation\u003c\/strong\u003e — The formulation contains no human- or animal-derived components, reducing the risk of introducing undefined biological materials during NK cell storage.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein-Free Cryopreservation Medium\u003c\/strong\u003e — Biofargo NKCryo contains no protein components, supporting applications requiring controlled cryopreservation conditions for immune cell research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized for Human NK Cell Freezing\u003c\/strong\u003e — Suitable for NK cells generated from peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cell sources.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-Density NK Cell Freezing Capability\u003c\/strong\u003e — Supports cryopreservation densities ranging from 1 × 10\u003csup\u003e6\u003c\/sup\u003e to 5 × 10\u003csup\u003e8\u003c\/sup\u003e cells\/mL, accommodating both routine laboratory storage and high-density immune cell banking workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSupports Post-Thaw NK Cell Recovery\u003c\/strong\u003e — Designed to minimize freezing-related stress and maintain high post-thaw cell viability and recovery after cryopreservation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow Endotoxin Cell Culture Grade\u003c\/strong\u003e — Manufactured with cell culture-grade raw materials and controlled endotoxin levels for reliable immune cell research applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with NK Cell Expansion Workflows\u003c\/strong\u003e — Can be integrated into workflows involving NK cell activation, expansion, freezing and subsequent recovery culture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\n\u003ch3\u003ePost-Thaw NK Cell Viability After Cryopreservation\u003c\/h3\u003e\n\n\u003cp\u003eCryopreservation performance was evaluated using human NK cells with a CD3\u003csup\u003e-\u003c\/sup\u003eCD56\u003csup\u003e+\u003c\/sup\u003e proportion greater than 90%. Cells were generated from cord blood-derived mononuclear cells and expanded using a Biofargo NK activation workflow before freezing with Biofargo NKCryo.\u003c\/p\u003e\n\n\u003cp\u003eThe evaluation demonstrated that Biofargo NKCryo supports high NK cell survival after cryopreservation. Under high-density freezing conditions, cells maintained post-thaw viability above 90% after 3 months of storage and maintained viability above 80% during a 6-month storage period.\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-img-container\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_6f789b82-ce13-4c9b-923b-357821d82192.jpg?v=1784102354\" alt=\"Post-thaw viability analysis of human NK cells preserved with Biofargo NKCryo NK cell cryopreservation medium\" loading=\"lazy\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003ePost-thaw viability analysis of human NK cells preserved with Biofargo NKCryo NK cell cryopreservation medium\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eNK Cell Recovery After Long-Term Storage\u003c\/h3\u003e\n\n\u003cp\u003eCell recovery analysis demonstrated that Biofargo NKCryo maintains a high recovery rate following extended cryopreservation. After 6 months of storage, frozen NK cells maintained more than 80% cell recovery after thawing.\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-img-container\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/3_679f343f-bc93-404a-8b60-53518925698a.jpg?v=1784102256\" alt=\"Human NK cell recovery rate after long-term storage using serum-free NK cell cryopreservation medium\" loading=\"lazy\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eHuman NK cell recovery rate after long-term storage using serum-free NK cell cryopreservation medium\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eRecommended Cryopreservation Workflow\u003c\/h2\u003e\n\n\u003cp\u003eBiofargo NKCryo provides a standardized workflow for laboratories performing human NK cell expansion, freezing and recovery experiments. The recommended process includes cell harvesting, cryoprotectant addition, controlled-rate freezing and transfer to long-term storage.\u003c\/p\u003e\n\n\u003ch3\u003eStep 1: NK Cell Collection\u003c\/h3\u003e\n\n\u003cp\u003eAfter completion of NK cell culture and expansion, collect NK cells by centrifugation and carefully remove the culture supernatant.\u003c\/p\u003e\n\n\u003ch3\u003eStep 2: Add Biofargo NKCryo Freezing Medium\u003c\/h3\u003e\n\n\u003cp\u003eSlowly add Biofargo NKCryo cryopreservation medium to the cell pellet. Gently resuspend the cells to achieve a uniform suspension.\u003c\/p\u003e\n\n\u003cp\u003eRecommended final freezing density:\u003c\/p\u003e\n\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e to 5 × 10\u003csup\u003e8\u003c\/sup\u003e cells\/mL\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStep 3: Controlled Cooling and Storage\u003c\/h3\u003e\n\n\u003cp\u003eTransfer prepared cryovials into a controlled-rate freezing container and place them at -80°C. For long-term storage, transfer cells to liquid nitrogen storage after approximately 12 hours.\u003c\/p\u003e\n\n\u003ch3\u003eStep 4: NK Cell Thawing and Recovery\u003c\/h3\u003e\n\n\u003cp\u003eThaw frozen NK cells using a 37°C water bath or cell recovery instrument. Transfer cells into a centrifuge tube and add at least three volumes of serum-free NK cell culture medium or physiological saline to dilute the cryopreservation solution. Centrifuge to remove the freezing medium before downstream experiments.\u003c\/p\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\n\u003cp\u003eBiofargo NKCryo Serum-Free NK Cell Cryopreservation Medium is designed for laboratories working with human natural killer cells, immune cell expansion platforms and cell-based research workflows requiring reliable frozen cell preservation.\u003c\/p\u003e\n\n\u003cp\u003eThe product supports applications where maintaining NK cell viability, recovery and consistency after thawing is important, including immune cell research, NK cell banking and cell therapy development workflows.\u003c\/p\u003e\n\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHuman NK Cell Cryopreservation\u003c\/strong\u003e — Supports freezing and storage of expanded human NK cells while maintaining post-thaw viability for further research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNK Cell Expansion and Cryopreservation\u003c\/strong\u003e — Suitable for workflows combining NK cell activation, expansion, harvesting and long-term frozen storage.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePBMC-Derived NK Cell Preservation\u003c\/strong\u003e — Designed for NK cells generated from peripheral blood mononuclear cell sources.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCord Blood NK Cell Cryopreservation\u003c\/strong\u003e — Applicable for research workflows using cord blood-derived NK cell populations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmune Cell Cryopreservation Research\u003c\/strong\u003e — Provides a specialized cryopreservation solution for immune cell-based laboratory studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCAR-NK Cell Research\u003c\/strong\u003e — Suitable for research workflows where expanded NK cell populations require freezing, storage and recovery before functional analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNK Cell Banking\u003c\/strong\u003e — Supports establishment of frozen NK cell stocks for repeated experimental use and longitudinal studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePost-Thaw NK Cell Functional Studies\u003c\/strong\u003e — Enables recovery of NK cells for downstream assays including phenotype analysis, expansion studies and immune function evaluation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eAdvantages for NK Cell Research Workflows\u003c\/h2\u003e\n\n\u003cp\u003eMaintaining consistent NK cell quality after freezing is an important consideration in immune cell research. Variations in freezing conditions, cryoprotectant formulations and cell density may influence recovery performance and experimental reproducibility.\u003c\/p\u003e\n\n\u003cp\u003eBiofargo NKCryo provides a dedicated NK cell freezing solution designed around the requirements of human NK cell preservation. Its serum-free, protein-free and animal component-free formulation helps researchers establish controlled cryopreservation workflows while supporting high-density NK cell storage.\u003c\/p\u003e\n\n\u003cp\u003eBy combining optimized cryoprotection, low endotoxin manufacturing control and compatibility with NK expansion workflows, Biofargo NKCryo helps laboratories reduce variability between fresh and frozen NK cell experiments.\u003c\/p\u003e\n\n\u003ch2\u003eExperimental Considerations\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eUse freshly harvested and healthy NK cells with appropriate viability before cryopreservation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMaintain recommended freezing density to achieve consistent cryopreservation performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse controlled-rate freezing procedures whenever possible to reduce freezing stress.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid repeated freeze-thaw cycles of cryopreserved NK cell samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEvaluate post-thaw viability and recovery before downstream functional assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Biofargo NKCryo NK Cell Cryopreservation Medium?\u003c\/summary\u003e\n\u003cp\u003eBiofargo NKCryo is a serum-free NK cell cryopreservation medium developed specifically for human natural killer cell freezing, storage and recovery. It is designed to reduce cellular stress during freezing, transportation and thawing while supporting post-thaw NK cell viability and recovery.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat type of cells can be preserved with Biofargo NKCryo?\u003c\/summary\u003e\n\u003cp\u003eBiofargo NKCryo is designed for human NK cell cryopreservation applications, including NK cells derived from peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cell sources.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eIs Biofargo NKCryo a serum-free NK cell freezing medium?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo NKCryo is a serum-free, animal component-free and protein-free cryopreservation medium. The formulation does not contain human- or animal-derived components, helping reduce variability associated with undefined biological materials.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eDoes Biofargo NKCryo contain DMSO?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo NKCryo contains 10% DMSO (v\/v), which provides cryoprotective activity during the freezing process.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat freezing density is recommended for Biofargo NKCryo?\u003c\/summary\u003e\n\u003cp\u003eBiofargo NKCryo supports high-density NK cell cryopreservation from 1 × 10\u003csup\u003e6\u003c\/sup\u003e to 5 × 10\u003csup\u003e8\u003c\/sup\u003e cells\/mL. The appropriate freezing density may depend on the experimental design and downstream application requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan Biofargo NKCryo be used for NK cell expansion and cryopreservation workflows?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo NKCryo can be incorporated into workflows involving NK cell activation, expansion, harvesting, cryopreservation and post-thaw recovery for research applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eHow long can NK cells be stored after freezing with Biofargo NKCryo?\u003c\/summary\u003e\n\u003cp\u003eFor long-term storage, NK cells frozen with Biofargo NKCryo can be transferred to liquid nitrogen storage after initial freezing. Performance evaluation demonstrated maintained cell viability and recovery during extended storage periods.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat post-thaw performance can be expected from Biofargo NKCryo?\u003c\/summary\u003e\n\u003cp\u003ePerformance evaluation showed that NK cells cryopreserved with Biofargo NKCryo maintained post-thaw viability above 90% after 3 months and above 80% after 6 months under tested storage conditions. Cell recovery after 6 months remained above 80%.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan Biofargo NKCryo support CAR-NK cell research workflows?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo NKCryo can support research workflows where expanded NK cell populations, including CAR-NK related research samples, require freezing, storage and recovery before downstream analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eHow should NK cells frozen with Biofargo NKCryo be thawed?\u003c\/summary\u003e\n\u003cp\u003eFrozen NK cells can be thawed using a 37°C water bath or cell recovery instrument. After thawing, add at least three volumes of serum-free NK cell culture medium or physiological saline, then centrifuge to remove the cryopreservation medium before further experiments.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat quality control tests are performed for Biofargo NKCryo?\u003c\/summary\u003e\n\u003cp\u003eBiofargo NKCryo is tested for endotoxin level, bacterial contamination and fungal contamination. The product is filtered through a membrane smaller than 0.2 μm and manufactured using cell culture-grade raw materials.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eHow should Biofargo NKCryo be stored?\u003c\/summary\u003e\n\u003cp\u003eBiofargo NKCryo should be stored at 2–8°C. The recommended shelf life is 12 months under proper storage conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eIs Biofargo NKCryo intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. Biofargo NKCryo is intended for research use only and is not intended for direct therapeutic, diagnostic or human administration applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003c\/div\u003e","brand":"XRbio","offers":[{"title":"100mL","offer_id":52805033296053,"sku":"XRNKCRYO-0-100","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d006d7f8-a23c-4f1e-9f56-8b8c385e65bd.jpg?v=1784102535","url":"https:\/\/biofargo.com\/products\/nkcryo-serum-free-nk-cell-cryopreservation-solution","provider":"Biofargo","version":"1.0","type":"link"}