Description

Nitrotetrazolium Blue Chloride (NBT) is a high-purity yellow crystalline dye widely used in biochemical and cell-based assays. NBT functions as an electron acceptor, forming a dark blue insoluble formazan upon reduction, making it a powerful tool for detecting enzymatic activity and cellular metabolic processes. Its reliable performance allows researchers to visualize and quantify enzyme reactions, particularly in histochemistry, cytochemistry, and microbiological studies.

Supplied as a yellow crystalline powder with high solubility in water (10 mg/mL), NBT enables consistent and reproducible results in both qualitative and quantitative assays. Its high purity (≥90% by HPLC) ensures minimal interference in experimental procedures, making it an essential reagent for laboratory research in enzymology, cell biology, and related fields.

Specifications

Item	Value
Product Name	Nitrotetrazolium Blue Chloride (NBT)
Grade	High Purity
Product Code	NDB0379
Molecular Formula	C40H30N10O6Cl2
Molecular Weight	817.64
CAS No.	298-83-9
Appearance	Yellow Crystalline Powder
Identification	Positive
Assay (HPLC)	≥90% (TLC)
Sulfate (ASH)	≤0.3%
Solubility (10 mg/mL, H₂O)	Clear Yellow Solution
Melting Point	≥184℃

Features

High Purity: ≥90% (HPLC), ensuring accurate and reproducible assay results.
Reliable Enzyme Detection: Forms dark blue formazan upon reduction, suitable for measuring enzymatic activity.
Water Soluble: Readily dissolves in water to form a clear yellow solution for experimental use.
Versatile Applications: Suitable for histochemical, cytochemical, microbiological, and biochemical studies.
Stable Yellow Crystalline Powder: Melting point ≥184°C, providing convenient storage and handling in the lab.

Applications

Enzyme Activity Assays: Visualize and quantify enzyme reactions in vitro.
Histochemical and Cytochemical Staining: Detect metabolic activity in tissue sections and cultured cells.
Microbiological Analysis: Identify microbial enzymatic activity and metabolic processes.
Cell Viability Studies: Assess cellular respiration and redox activity in research assays.
Educational and Analytical Use: Demonstrate enzyme-mediated color changes in teaching laboratories.

Documents

COA

SDS

Disclaimer: For laboratory research use only.

Nitrotetrazolium Blue Chloride (NBT) 90% High Purity Grade