Description

The Ni-NTA 6FF Pre-Packed Gravity Column (1 mL) features an advanced Ni-NTA sefinose resin, which is a 6% cross-linked agarose resin covalently coupled to nitrilotriacetic acid (NTA). This resin forms high-affinity complexes with Ni²+ ions through three coordination sites, enabling highly efficient purification of His-tagged recombinant proteins. The resin is ideal for protein purification under both native and denaturing conditions, and it is compatible with various recombinant expression systems, including E. coli, yeast, insect cells, and mammalian cells.

Elution of bound His-tagged proteins is possible with low pH buffers, imidazole, or even histidine solutions, making it a versatile choice for your protein purification needs.

Specification

Item	Value
Package Size	Available in 5-pack or 10-pack
Substrate	6% Cross-linked Agarose
Average Particle Size	45 μm - 165 μm
Protein Binding Capacity	Approximately 10-40 mg of His-tagged protein per 1 mL resin
Maximum Flow Rate	150 cm/h
Pressurization	0.3 MPa (3 bar)
Storage Conditions	2-8°C, do not freeze. Store in 20% ethanol solution

Features

High Purity Protein Separation: The 6% cross-linked agarose and NTA matrix provide superior affinity and stability, reducing non-specific binding for higher purity target proteins.
Refolding of Denatured Proteins: Supports protein refolding, allowing simultaneous purification and renaturation of denatured proteins from inclusion bodies.
Broad Chemical Compatibility: Stable in all commonly used buffers, detergents, and denaturants. Avoid using chelating agents such as EDTA, EGTA, and citrate for optimal performance.
Wide pH Stability: The resin remains stable in a pH range of 2-14 (short-term, up to 2 hours), ensuring reliable performance under diverse conditions.
Minimal Ni²+ Leakage: The Ni-NTA resin minimizes Ni²+ leakage during purification, maintaining binding capacity and enhancing reproducibility.
Reusability & Cost-Effectiveness: The column can be regenerated and reused 5-10 times, offering an economical solution for high-throughput protein purification workflows.

Applications

His-Tagged Protein Purification: Ideal for purifying recombinant His-tagged proteins from various expression systems including bacteria, yeast, insect cells, and mammalian cells.
Protein Refolding: Suitable for denatured proteins, enabling simultaneous separation and refolding.
Protein Interaction Studies: Perfect for capturing His-tagged proteins for further structural and functional studies.
Biotechnological and Pharmaceutical Research: Essential for recombinant protein production, antibody purification, and enzyme activity assays in industrial and research settings.

Storage

Storage Temperature: Store at 2-8°C; do not freeze.
Storage Buffer: The resin is preserved in 20% ethanol to maintain activity.

Documents

User Manual

Ni-NTA 6FF(Pre-Packed Gravity Column),1mL