Natural Killer Cells Induction Culture Kit 2.0, BioSci

Product Introduction:

The Natural Killer Cells Induction Culture Kit 2.0 is a culture kit for in vitro culture of NK cells developed in GMP confirming processes, which contains N500 Serum Free Medium for NK Cells, NK cell activator and NK cell stimulator. This product is a chemically defined, all the compositions of which are produced from medicinal/cell culture grade materials. This product contains no animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research. The optimized formula of this product can significantly improve the proportion, the viability and the killing rate of NK cells in cells cultured in vitro.

Packing Specification:

Product Details:

• During blood collection, heparin or sodium citrate anticoagulant can be used, but EDTA anticoagulant cannot be used.
• The blood collection volume can be estimated based on 1×10⁶ PBMCs per milliliter of peripheral blood.
• Place the medium at room temperature for equilibrium or extract an estimated amount, and reheat to 37℃.
• If the temperature of the medium is too low or the cell density is too high, flocculated cells may appear, and the cell viability is reduced.
• Cell passage shall be carried out gently to avoid mechanical damages to cells.
• The amplification medium for NK cells is valid for three weeks.
• Use up the NK cell stimulator and NK cell activator at one time, and it shall not be frozen and thawed repeatedly, and shall be used within the validity of the product. After the complete medium is prepared by mixing, it is recommended to be stored away from light at 2℃ to 8℃ and used up within two weeks, and attention shall be paid to operate in sterile conditions.
• Application: Research use only.
• The culture example is only for reference, and the optimization of the culture procedure is determined by users based on experience.

Documents:

Technical Data Sheet