Description

Paralichthys olivaceus-Ingredient Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Compatible with various DNA purification methods
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
2×Probe qPCR MasterMix	981201	0.5 mL	0.5 mL Vial	Natural
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-242	50 T	0.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc242	50 μL	0.5 mL Vial	Yellow
Ultrapure water	210806	1 mL	1.5 mL Vial	Blue
Manual	sc15-51900	1 Set	NA	NA

Application Areas

Detection of Paralichthys olivaceus-Ingredient in marine and aquaculture samples
Health monitoring in abalone farming and marine biology research
Ideal for disease surveillance, food safety research, and ecological monitoring

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Documents

Paralichthys olivaceus-Ingredient Probe qPCR Kit User Manual