Description

The MTT Assay Reagent provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents. Among all non-radioactive viability assays, MTT assay developed by Mossman is one of the most versatile and popular assays.

MTT is a tetrazolium salt that is turned into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells, not in dead cells. The amount of formazan product generated is proportional to the number of living cells in the sample. At the end of the assay, the cells containing the formazan product are solubilized and then photometrically quantified at 570 nm.

Features

Ready-to-Use Format

Supplied as a convenient reagent that simplifies assay setup and minimizes preparation time.
Reliable Cell Viability Measurement

Quantifies metabolically active cells based on mitochondrial enzyme activity.
Non-Radioactive and Widely Validated

One of the most commonly used colorimetric viability assays in cell biology research.
Quantitative and Reproducible Results

Formazan production correlates directly with the number of living cells.
Compatible with Standard Plate Readers

Absorbance measurement at 570 nm enables easy integration into routine workflows.

Applications

Cell proliferation assays
Cytotoxicity and drug screening studies
Cell viability assessment
Metabolic activity analysis
Cancer biology and pharmacology research

Documents

Data Sheet

MSDS

MTT Assay Reagent, Ready-to-use