Description

Due to the structure and properties of the mouse tail itself, the extraction of mouse tail genomic DNA has always troubled researchers: one is that the processing time of the mouse tail is long and the genomic DNA is easily degraded, the other is difficult to handle and the purity of the extracted genomic DNA is low.

This kit uses a DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can digest mouse tail samples within 45 minutes, thereby minimizing the degradation of genomic DNA and shortening sample processing time. The kit can extract 10-20μg of high-quality, high-purity genomic DNA from 0.5-1cm juvenile or adult mouse tails within 60-90min.

The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in the cell. The extracted genomic DNA fragment is large, high in purity, stable and reliable in quality, and the size of the DNA fragment is stable at about 23kb.

The kit can also be used to extract other mouse tissues outside the mouse tail, including mouse ears, mouse muscle, mouse liver and other tissues, so that one kit can be used for multiple purposes and meet the needs of users for different experiments.

Features

No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
Safety: No organic reagent extraction is required.
High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Application

It is suitable for the extraction and purification of genomic DNA from fresh or frozen large and mouse tails (also used to purify mouse or rat muscle and mouse ear genomic DNA).

Genomic DNA Application:

The genomic DNA purified by the Mouse Tail DNA Mini Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.

Work Flow

Storage

This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
Foregene Protease Plus solution has a unique formula, it is active when stored at room temperature for a long time (3 months); stored at 4°C, the activity and stability will be better, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

Documents

Mouse Tail DNA Mini Kit Instruction Manual-V1.0-EN