Bcakground

Stem cell factor (SCF) , also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

Reference

1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.

2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.

3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.

4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.

5. Kapur, R. et al. (2002) Blood 100:1287.

6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.

7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.

8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.

9. Huang, E. et al. (1990) Cell 63:225.

10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.

11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.

12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.

13. Flanagan, J.G. et al. (1991) Cell 64:1025.

14. Martin, F.H. et al. (1990) Cell 63:203.

15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Specifications

Synonyms	Mast cell growth factor; MGF; MGFSHEP7; SCF;c-kit Ligand; DCUA; DFNA69; Kitl; KITLG
Accession #	P20826
Source	Human embryonic kidney cell, HEK293-derived mouse SCF/c-kit Ligand protein
Source	Lys26-Ala189
Predicted Moleucular weight	18.3 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells
Activity	The EC50 for this effect is 0.5-3 ng/mL.

SDS-PAGE

Bioactivity

Documents

Mouse SCF