Bcakground

TRANCE/TRANCE/RANK L is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

Reference

1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.

2. Anderson, D.M. et al. (1997) Nature 390:175.

3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

4. Kong, Y-Y. et al. (1999) Nature 397:315.

Specifications

Synonyms	CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; RANK L; RANKL
Accession #	O35235
Source	Human embryonic kidney cell, HEK293-derived mouse RANKL protein
Source	Arg72-Asp316
Predicted Moleucular weight	27.6 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE.
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method.
Activity	Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells.
Activity	The EC50 for this effect is 0.2-1 ng/mL

SDS-PAGE

Bioactivity

Documents

Mouse RANKL