Bcakground

Macrophage Colony Stimulating Factor(M-CSF),also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (9, 10). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Reference

1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.

2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.

3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.

4. Ryan, G.R. et al. (2001) Blood 98:74.

5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.

6. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.

7 Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

Specifications

Synonyms	colony stimulating factor 1 (macrophage); CSF1; CSF-1; MCSF; M-CSF
Accession #	P07141
Source	Human embryonic kidney cell, HEK293-derived Mouse M-CSF protein
Source	Lys33-Glu262
Predicted Moleucular weight	26 kDa(Monomer)
Form/Structure	Dimer in solution

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice.

Quality

Purity	> 95%, determined by SDS-PAGE.
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells.
Activity	The EC50 for this effect is 0.2-1.5 ng/mL.

SDS-PAGE

Gel filtration

Bioactivity

Documents

Mouse M-CSF