Description

Establishing and maintaining stable mouse lung organoid models can be challenging during three-dimensional cell culture workflows. Variations in tissue processing, culture conditions, and undefined components may influence organoid formation efficiency, expansion capacity, and experimental reproducibility. Researchers studying pulmonary biology require a reliable culture system that supports consistent lung-derived organoid growth while reducing workflow complexity.

XR Mouse Lung Organoid Culture Kit (XRmLungO-100) is a serum-free, defined culture medium system designed for the establishment, maintenance, and expansion of mouse lung organoids. The complete mouse lung organoid culture system consists of a specialized basal medium and optimized culture supplement to support three-dimensional lung organoid culture workflows.

This mouse lung organoid medium provides researchers with a standardized solution for generating and expanding lung-derived organoid models from mouse tissue. The defined formulation helps improve consistency between experiments and supports applications requiring reliable pulmonary research models, including lung development studies, respiratory disease modeling, and organoid-based research workflows.

Specifications

Product Name	XR Mouse Lung Organoid Culture Kit
SKU	XRmLungO-100
Product Type	Mouse lung organoid culture medium system
Components	Mouse Lung Organoid Basal Medium + Mouse Lung Organoid Culture Supplement
Basal Medium Volume	96 mL
Culture Supplement Volume	4 mL
Culture Format	3D lung organoid culture
Formulation	Serum-free and defined culture system
Recommended Culture Conditions	37°C, 5% CO₂
Storage	Basal Medium: 4°C or -20°C Supplement: -20°C
Research Grade	Research Use Only (RUO)

Features

Specialized Mouse Lung Organoid Culture System — Designed specifically for establishment and expansion of mouse lung-derived organoids in three-dimensional culture workflows.
Serum-Free Defined Medium Formulation — Provides a controlled culture environment to reduce variability associated with serum-containing systems.
Complete Lung Organoid Expansion Solution — Includes optimized basal medium and supplement for convenient organoid culture preparation.
Supports 3D Organoid Growth — Compatible with Matrigel-based organoid culture workflows requiring extracellular matrix-supported growth.
Designed for Pulmonary Research Applications — Suitable for mouse lung development studies, respiratory biology research, and disease model establishment.
Improved Workflow Consistency — Defined components help researchers achieve more reproducible organoid culture conditions.

Performance Data

Mouse Lung Organoid Formation and Expansion

XR Mouse Lung Organoid Culture Kit was evaluated for its ability to support mouse lung tissue-derived organoid formation and expansion during three-dimensional culture. Following tissue dissociation and extracellular matrix embedding, mouse lung-derived cells were cultured using XR mouse lung organoid medium under standard organoid culture conditions.

During the culture process, progressive organoid development was observed from initial cell aggregation to organized three-dimensional structures. The results demonstrate that XRmLungO-100 provides a suitable environment for mouse lung organoid establishment and subsequent expansion.

Mouse lung organoid formation and expansion using XR lung organoid culture medium

Representative images show different stages of mouse lung organoid growth, including early culture stage, organoid formation, and expanded organoid structures.

Mouse Lung Organoid Morphology During Culture

Three-dimensional morphology is an important characteristic of organoid culture systems. XR Mouse Lung Organoid Culture Kit supports the formation of spherical and structured lung organoid models suitable for downstream pulmonary research applications.

Three dimensional mouse lung organoid morphology cultured with serum free organoid medium

The generated organoid structures can be further applied for characterization studies, pathway analysis, and experimental models requiring physiologically relevant lung tissue-like systems.

Lung Organoid Marker Characterization

Mouse lung organoid identity and cellular characteristics can be evaluated using commonly used lung epithelial markers. XR Mouse Lung Organoid Culture Kit supports maintenance of lung-associated cellular features during organoid culture.

Immunofluorescence analysis demonstrates expression of lung-associated markers including KRT5 and SPC within cultured mouse lung organoid structures, supporting the use of this system for pulmonary organoid research.

Applications

XR Mouse Lung Organoid Culture Kit is designed for researchers requiring a reliable mouse lung organoid culture medium system for three-dimensional pulmonary research models. The serum-free defined formulation supports workflows involving organoid establishment, maintenance, expansion, and downstream analysis.

Mouse Lung Organoid Culture — Establishment and expansion of mouse lung-derived organoids from primary tissue sources.
Lung Development Research — Investigation of pulmonary development, epithelial organization, and lung progenitor cell behavior using three-dimensional culture models.
Pulmonary Disease Modeling — Generation of in vitro lung disease models for studying disease mechanisms and cellular responses.
Respiratory Research — Supports research involving airway biology, lung epithelial function, and respiratory system-related studies.
Drug Screening and Compound Evaluation — Provides a physiologically relevant organoid platform for evaluating compound responses and biological effects.
Lung Regeneration Research — Enables studies focused on tissue repair, epithelial regeneration, and organoid-based regenerative biology.
Cell Signaling and Molecular Mechanism Studies — Suitable for investigating pathways regulating lung cell growth, differentiation, and organization.
Three-Dimensional Cell Culture Research — Compatible with organoid workflows requiring extracellular matrix-supported 3D culture environments.

Why Choose XR Mouse Lung Organoid Culture Kit?

Developing reproducible lung organoid models requires more than simply maintaining cell survival. Researchers need culture conditions that support tissue-specific organization, consistent expansion, and reliable downstream analysis. Conventional cell culture media may not provide the specialized environment required for long-term three-dimensional lung organoid workflows.

XR Mouse Lung Organoid Culture Kit provides a dedicated mouse lung organoid medium system combining basal medium and optimized supplement components. The serum-free defined formulation helps reduce experimental variation while supporting organoid establishment and expansion for pulmonary research applications.

Compared with general-purpose cell culture media, this specialized lung organoid culture system is designed around the requirements of mouse lung tissue-derived organoid workflows, helping researchers build more consistent in vitro lung models for disease research, drug discovery, and regenerative biology studies.

Workflow Overview

The mouse lung organoid culture workflow includes tissue dissociation, cell preparation, extracellular matrix embedding, and culture expansion using XR mouse lung organoid complete medium.

Technical Information

Sample Source	Mouse lung tissue
Culture Model	Three-dimensional lung organoid model
Matrix Compatibility	Matrigel-based organoid culture workflow
Culture Environment	37°C, 5% CO₂ incubator
Application Category	Organoid culture, pulmonary research, tissue modeling
Product Use	Research use only

FAQ

What is XR Mouse Lung Organoid Culture Kit used for?

XR Mouse Lung Organoid Culture Kit is a specialized mouse lung organoid culture medium system designed for the establishment, maintenance, and expansion of mouse lung-derived organoids in three-dimensional culture workflows. It is intended for pulmonary research applications including lung development studies, disease modeling, and organoid-based research.

What type of organoid culture does this product support?

This product supports mouse lung organoid culture using a three-dimensional extracellular matrix-based culture workflow. The system is designed to help researchers generate and expand lung tissue-derived organoid models under controlled culture conditions.

Is XR Mouse Lung Organoid Culture Kit a serum-free culture system?

Yes. XR Mouse Lung Organoid Culture Kit uses a serum-free, defined culture formulation designed to provide a controlled environment for mouse lung organoid establishment and expansion while reducing variability associated with serum-containing culture systems.

What components are included in the mouse lung organoid culture kit?

The XRmLungO-100 kit contains a mouse lung organoid basal medium and a mouse lung organoid culture supplement. These components are supplied together as a complete mouse lung organoid culture system and are not sold separately.

Can this product be used for lung disease modeling research?

Yes. Mouse lung organoid models generated with this culture system can be used as in vitro pulmonary research platforms for studying lung biology, respiratory disease mechanisms, cellular responses, and related disease modeling applications.

What are the advantages of using a mouse lung organoid model compared with traditional 2D cell culture?

Three-dimensional mouse lung organoid culture provides a more tissue-relevant experimental model compared with conventional two-dimensional cell culture. Organoid systems allow researchers to study cellular organization, growth behavior, and biological responses within a more complex microenvironment.

What culture conditions are recommended for XR Mouse Lung Organoid Culture Kit?

Mouse lung organoid cultures using XRmLungO-100 are typically maintained under standard mammalian cell culture conditions at 37°C with 5% CO₂. Specific experimental conditions may require optimization according to tissue source and research objectives.

How should XR Mouse Lung Organoid Culture Kit be stored?

The mouse lung organoid basal medium can be stored at 4°C for short-term storage or -20°C for extended storage. The culture supplement should be stored at -20°C according to the product specification.

Research Use Statement

XR Mouse Lung Organoid Culture Kit is supplied for Research Use Only (RUO). This product is intended for laboratory research applications involving mouse lung organoid culture, three-dimensional cell culture, and pulmonary research workflows. It is not intended for diagnostic, therapeutic, or clinical use.