Bcakground

Leptin is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development.

Specifications

Synonyms	LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein.
Accession #	P41160
Source	Human embryonic kidney cell, HEK293-derived mouse Leptin protein
Source	Val22-Cys167
Predicted Moleucular weight	41.8 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening
Storage and Stability	Avoid repeated freeze-thaw cycles
	It is recommended that the protein be aliquoted for optimal storage
	12 months from date of receipt, -20 to -70 as supplied
Shipping	Shipping with dry ice

Quality

Purity	≥95%, determined by SDS-PAGE
Endotoxin Level	＜0.01 EU per 1 µg of the protein by the LAL method
Activity	Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human Leptin R. The EC50 for this effect is 0.075-0.45 ng/mL

SDS-PAGE

Bioactivity

Documents

Mouse LEPTIN