Bcakground

Interleukin-5 (IL-5)is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 - 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for mouse IL-5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL-5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 - 3, 6 - 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal-transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12).

Reference

1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.

3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.

4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.

5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.

6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.

7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.

8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.

9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.

10. Cameron, L. et al. (2000) J. Immunol. 164:1538.

11. Tavernier, J. et al. (1991) Cell 66:1175.

12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.

Specifications

Synonyms	IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5
Accession #	P04401
Source	Human embryonic kidney cell, HEK293-derived mouse IL-5 protein
Source	Met21-Gly133
Predicted Moleucular weight	13.1 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
Activity	The EC50 for this effect is 17-35.6 pg/mL.

SDS-PAGE

Bioactivity

Documents

Mouse IL5