Bcakground

Interleukin 1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a widevariety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, theybind to the same receptor and exert identical biological effec(ts1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by thecysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

Reference

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.

2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.

3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.

4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.

5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.

6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Specifications

Synonyms	IL1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1-beta
Accession #	NP_032387
Source	Human embryonic kidney cell, HEK293-derived mouse IL-1 beta/IL-1F2 protein
Source	Val118-Ser269
Predicted Moleucular weight	17.5 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening
Storage and Stability	Avoid repeated freeze-thaw cycles
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice.

Quality

Purity	> 95%, determined by SDS-PAGE.
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells
Activity	The EC50 for this effect is 1-4 pg/mL

SDS-PAGE

Gel filtration

Bioactivity

Documents

Mouse IL1b