Bcakground

Interleukin-17A(IL-17A) , also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL -17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15,16). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).

Reference

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.

2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.

3. Yao, Z. et al. (1996) Gene 168:223.

4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.

6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.

7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.

8. Rong, Z. et al. (2009) Cell Res. 19:208.

9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.

10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.

11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.

12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.

13. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.

14. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.

15. Hsu, H. et al. (2008) Nat. Immunol. 9:166.

16. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.

17. Wang, L. et al. (2009) J. Exp. Med. 206:1457.

18. Kryczek, I. et al. (2009) Blood 114:357.

Specifications

Synonyms	IL17; IL-17; IL17A; IL-17A; CTLA8; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8
Accession #	Q62386
Source	Human embryonic kidney cell, HEK293-derived mouse IL-17/IL-17A protein
Source	Ala26-Ala158
Predicted Moleucular weight	15.0 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells
Activity	The EC50 for this effect is 0.12-1.25 ng/mL.

SDS-PAGE

Bioactivity

Documents

Mouse IL17A