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Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

Reference

1. Pestka, S. (2007) J Biol Chem. 282:20047.

2. Paul, F. et al. (2015) Gene. 567(2):132.

3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.

4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.

5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.

6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Specifications

Synonyms	alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2
Accession #	P01573
Source	Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein
Source	Cys24-Glu190
Predicted Moleucular weight	19.4 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus
Activity	The EC50 for this effect is 20-200 pg/mL.

SDS-PAGE

Bioactivity

Documents

Mouse IFNA2