Bcakground

Hepatocyte Growth Factor(HGF) , also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14).

Reference

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.

2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.

3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.

4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.

5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.

6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.

7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.

8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.

9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.

10. Montesano, R. et al. (1991) Cell 67:901.

11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.

12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.

13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.

14. Mineo, R. et al. (1994) Endocrinology 145:4355.

Specifications

Synonyms	F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF
Accession #	Q08048
Source	Human embryonic kidney cell, HEK293-derived mouse HGF protein
Source	Gln33-Arg495 (alpha) & Val496-Leu728 (beta)
Predicted Moleucular weight	79.3kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles
	It is recommended that the protein be aliquoted for optimal storage
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells.
Activity	The EC50 for this effect is 1-10 ng/mL.

SDS-PAGE

Bioactivity

Documents

Mouse HGF