Bcakground

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells (5). The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members.FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

Reference

1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.

2. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

3. Presta, M. et al. (2005) Cytokine. Growth Factor Rev. 16:159.

4. Claus, P. et al. (2003) J. Biol. Chem. 278:479.

5. Quarto, N. et al. (2005) Gene 356:49.

6. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Comm. 335:1239.

7. Hebert, J. M. et al. (1990) Dev. Biol. 138:454.

Specifications

Synonyms	basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2
Accession #	P15655
Source	Human embryonic kidney cell, HEK293-derived mouse FGF basic/FGF2/bFGF protein
Source	Ala11-Ser154
Predicted Moleucular weight	16.2 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells
Activity	The EC50 for this effect is 0.01-0.5 ng/mL

SDS-PAGE

Bioactivity

Documents

Mouse FGF2