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Description
This kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect residual host cell proteins (HCPs) from MDCK cells. A sheep polyclonal antibody specific to MDCK HCPs was employed in the assay to capture any remaining HCPs in the sample. The antibody coverage is assessed by the current mainstream method. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled with anti-MDCK HCP antibody were simultaneously added to the microtiter plate coated with the affinity purified capture antibody, and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength was positively correlated with the HCPs concentration in the calibration standard and the samples. The concentration of HCPs in the samples can be calculated using a dose-response curve.
| Components | Shipping Condition | Unit Size | Detection Method |
| MDCK HCP Calibration Standard | 2-8℃ | 96 Tests | Sandwich ELISA |
| Anti-MDCK HCP Microtiter Strips | |||
| Reconstitution Solution | |||
| Diluent | |||
| Wash Buffer Concentrate (10×) | |||
| Anti-MDCK: HRP (100×) | |||
| TMB Substrate | |||
| Stop Solution | |||
| Sealing Film |
Specifications
| Linearity & Range | 2-200 ng/mL, R2>0.990 |
| Accuracy | Recovery Rate= 95.8%-110.8% |
| LLOQ | 2 ng/mL |
| ULOQ | 200 ng/mL |
| LOD | 2 ng/mL |
| Precision | 1.6%-5.5% (CV≤20%) |
| Antibody Coverage (IMBS-2D) | 74.2%-89.7% |
| Antibody Coverage (IMBS-LC/MS) | 70.10% |
| Specificity | No cross-reactivity with E.coli, P.pastoris and Sf9 cells. |
| Robustness | Incubate at 25±5℃, CV≤ 20% |
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Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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