Composition

Name	Article Number	Specification	Storage Conditions	Shelf Life
MaxSortin® CD138 Separation Magnetic Beads	TL-811-1000	1 mL for 1×10⁹ total cells	2 - 8 °C	6 months
MaxSortin® Cell Sorting Buffer	MS-BF100	100 mL	2 - 8 °C	12 months
MaxSortin® L-Separation Column	MS-CL01	1 piece	10 - 35 °C	12 months

Specification

Reactive Species	Human
Endotoxin	<2EU/mL
Appearance	Brown liquid

Intended Use

MaxSortin® CD138 Separation Magnetic Beads can be used to sort CD138+ cells. By coupling anti-human CD138 monoclonal antibodies onto the magnetic beads, incubating them with cells, and then performing magnetic separation, the sorting of CD138+ cells can be achieved.

Experimental Procedures

Resuspend the mixed cell sample of PBMCs containing about 10% U266 in PBS buffer containing 1% human serum albumin (HSA). Take a sample for counting and transfer 1×10⁷ cells into a 1.5 mL Ep tube. Then centrifuge at 1500 rpm for 5 minutes.
Discard the supernatant, take 90 μL of MaxSortin® Cell Sorting Buffer solution to resuspend the cells, add 10 μL of MaxSortin® CD138 Separation Magnetic Beads, mix well and then place them in a refrigerator at 2 - 8 °C for incubation for 15 minutes.
Take the MaxSortin® L-Separation Column and place it on the magnetic-activated cell sorting (MACS) sorter, and rinse it twice with 1 mL of MaxSortin® Cell Sorting Buffer.
Take the sample that has completed incubation out of the refrigerator at 2 - 8 °C, add 1 mL of MaxSortin® Cell Sorting Buffer, centrifuge at 1500 rpm for 5 minutes, and discard the supernatant.
Add 1 mL of MaxSortin® Cell Sorting Buffer to resuspend the cells, add the sample into the MaxSortin® L-Separation Column. After it flows out naturally, add MaxSortin® Cell Sorting Buffer in two portions, 3 mL each time, to wash the cells adhering to the column.
After all the MaxSortin® Cell Sorting Buffer has flowed out, remove the MaxSortin® L-Separation Column from the MACS sorter and place it in a 15 mL centrifuge tube (mark it properly). Add 3 mL of MaxSortin® Cell Sorting Buffer into the MaxSortin® L-Separation Column and use the piston to push the liquid out directly. The collected cells are the CD138+ cells.
Count the cells and conduct flow cytometry detection.

Precautions

When incubating the magnetic beads with cells, it is necessary to mix them thoroughly to improve the sorting efficiency.
When the proportion of CD138+ cells is below 3%, it is recommended to add 5 μL of magnetic beads. When the proportion of CD138+ cells is above 3%, it is recommended to add 10 μL of magnetic beads.
This product cannot be used for clinical treatment.

MaxSortin® CD138 Isolation Kit