Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase

Description

RT Easy II(with gDNase) Master Premix system of this kit can also maintain good activity at 50°C. Therefore, it is more advantageous for complex RNA templates and can easily transcribe RNA templates with high GC content and complex secondary structure.

RT EasyTM II (With gDNase) is a reverse transcription system specially developed for Real Time PCR to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.

5× RO-EasyTM Mix contains Foregene Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase with stronger RNA affinity. The optimized system makes the reverse transcription rate faster, and the first strand cDNA synthesis can be completed at 42°C in 15 minutes

Kit components

• 5×gDNase Mix

• 5×RO-EasyTM Mix

• RNase-Free ddH2O

• Instructions

Features

• High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.

• The reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.

• Efficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.

• Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.

• Complex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.

Applications

• Used directly in Real Time PCR to quantitatively analyze gene expression.

• It can quickly and accurately analyze trace RNA such as RNA virus.

• Reverse transcription of RNA templates with high GC content or complex secondary structure.

Storage

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

Documents

RT Easy™ II (With gDNase) Instruction Manual-V1.0