{"product_id":"magnetic-hi-tissue-cell-total-rna-kit","title":"Magnetic Hi-Tissue\/Cell Total RNA Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe kit adopts magnetic beads and a unique buffer system, to isolate and purify total RNA with high quality from cell\/tissue. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it.\u003c\/p\u003e\n\u003cp\u003eIt is compatible with automated nucleic acid extractors. Through absorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient, and the extracted RNA has high purity. If high throughput automated extraction is required, Biofargo can deliver integrated solutions.\u003c\/p\u003e\n\u003cp\u003eRNA purified by this kit is suitable for downstream experiments including RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning.\u003c\/p\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eGroup\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eGDP771 (96 preps)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"10\" style=\"text-align: center;\"\u003eGDP771H\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNAstore Reagent\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer TSA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e70 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer ICBP\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e35 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMagAttract Suspension BE (100 mg\/ml)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer RDC\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e90 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer RW1A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e200 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer RW4\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e90 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBuffer RW\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNase-Free ddH₂O\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003eGRT431\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNase-Free DNase I (1500 U)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNase-Free ddH₂O\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: GRT431, GDP771H are shipped and stored separately.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSimple and fast: Ultra-pure total RNA can be obtained quickly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSafe and non-toxic: No toxic reagents such as phenol\/chloroform.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh purity: The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReagents need to be prepared by Customer\u003c\/h2\u003e\n\u003cp\u003eIsopropanol (supplementary protocol: samples preserved in Trizol), anhydrous ethanol, homogenization equipment (mortar, homogenizer, etc.), liquid nitrogen, gloves, masks, RNase-Free centrifuge tubes, magnetic racks, or nucleic acid extractor, for other brands of extractor to be integrated, please contact Biofargo.\u003c\/p\u003e\n\u003ch2\u003eRecommended dosage for different types of samples\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Please refer to the recommended ranges in the table below. Do not exceed 100 mg of tissue, otherwise it may lead to RNA yield and quality decrease.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eCategory\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSample Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eOptimal Extraction Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"12\" style=\"text-align: center;\"\u003eTissue\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSpleen\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eLiver\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eKidney\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntestine\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eGland\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eHeart\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eLung\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBrain\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFat\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSkin\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMuscle\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTail\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.3 cm (rat), 0.6 cm (mouse)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003eCell\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNon-stop protocol\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10⁵ – 10⁷ cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eLow yield, pause protocol recommended\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLess than 10⁵ cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003eInsects\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMollusc\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCrustacea\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align: center;\"\u003eFungus\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eYeast\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNot more than 5 × 10⁷ cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMould\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMushrooms\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e75 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAll components of the kit GDP771H can be stored in dry conditions at room temperature (15-30°C) for 15 months. RNase-Free DNase I and RNase-Free ddH2O can be stored at 2-8°C for 15 months.\u003c\/p\u003e\n\u003ch2\u003eImportant Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReplace the gloves with new ones frequently. This is because skin often carries bacteria that can lead to RNase contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid cross-contamination by using RNase-free plastics and tips.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePay attention to optimal sample storage and pretreatment conditions to avoid causing degradation of the extracted RNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePreparation of DNase I storage solution\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eDissolve DNase I dry powder (1500 U) in 1 ml RNase-Free ddH2O, mix gently and follow the protocol to add 10 μl to each sample for freshly use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAttention:\u003c\/strong\u003e If long-term storage needed, dissolve DNase I dry powder (1500 U) in 550 μl RNase-Free ddH2O to make stock solution, mix gently, dispense and store at -30~-15°C (can be stored for 9 months). Add 5 μl stock solution to each sample. Store the DNase I stock solution after thawing from -30~-15°C at 2-8°C (can be stored for 6 weeks) and do not freeze it again.\u003c\/p\u003e","brand":"Tiangen","offers":[{"title":"96preps","offer_id":52455088226485,"sku":"GDP771","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TIANGEN_1.png?v=1764815072","url":"https:\/\/biofargo.com\/products\/magnetic-hi-tissue-cell-total-rna-kit","provider":"Biofargo","version":"1.0","type":"link"}