Description

Bio Basic’s Lysozyme for Molecular Biology is a high-purity, triple-crystallized enzyme designed for efficient cell wall lysis, particularly in Gram-positive bacteria. With a protein content of ≥90% and specific activity ≥20,000 units per milligram, this lyophilized preparation is ideal for DNA extraction, structural biology, and proteomics workflows. The enzyme hydrolyzes the β(1→4) glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls, and is also effective on chitosan oligomers.

Specifications

Grade	Biotech
CAS	12650-88-3
Appearance	White to off-white granulate
Solubility (2%-aqua at 640nm)	≥95%
Purity on dry basis	≥95%
Activity in FIP Units/mg protein	≥39000
pH (2%-aqua)	3.0-3.6
Water content	≤6%
Residue on ignition	≤1.5%
Storage	-20℃

Applications

Bacterial cell lysis for plasmid DNA extraction
Structural and functional studies of proteins
Standard protein for mass spectrometry calibration
Compatible with boiling lysis protocols

Solubility

Soluble at 10 mg/mL in distilled water; forms a clear or slightly hazy solution. Best reconstituted in 10 mM Tris-HCl, pH 8.0.

Storage

Lyophilized: Stable for ≥4 years at -20°C
Reconstituted (pH 4–5): Stable for weeks at 2–8°C

Documents

INFO

COA

SDS

Disclaimer: For laboratory research use only.