Description

Luciferase-Expressing Rat Primary Dermal Epithelial Cells from Cell Biologics are isolated from the skin tissues of 6-8 weeks old laboratory Sprague–Dawley rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Luciferase-Expressing Rat Primary Dermal Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (sc-8426, Santa Cruz) or ZO-1 (MABT11, clone R40.76, Millipore) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Luciferase, distinct from GFP/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.

Suggested Medium

M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6066Luc

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.