Description

Luciferase-Expressing Human Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from Kidney Glomerular tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Luciferase-Expressing Human Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31/PECAM-1 (Catalog No. FAB3567P, R&D), or VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Luciferase, distinct from GFP/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.

Suggested Medium

H1168 Complete Human Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

H-6014G.Luc

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at

http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.