{"product_id":"lowry-protein-assay-kit","title":"Lowry Protein Assay Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLowry Protein Assay Kit is a classical protein quantification assay designed for accurate measurement of total protein concentration in biological samples. During protein research workflows, inconsistent protein input amounts can affect downstream experiments such as Western blot, SDS-PAGE, immunoprecipitation, and biochemical analysis. Reliable protein concentration determination is therefore essential for sample normalization and experimental reproducibility.\u003c\/p\u003e\n\u003cp\u003eThis Lowry Protein Assay Kit utilizes the Folin-Ciocalteu reagent-based colorimetric protein assay method. Under alkaline conditions, proteins react with copper ions to form protein-copper complexes, which subsequently reduce Folin reagent and generate a blue color signal. The color intensity is proportional to protein concentration and can be quantified using a microplate reader or spectrophotometer.\u003c\/p\u003e\n\u003cp\u003eWith a detection range of 5–500 μg\/mL protein, this total protein assay kit provides a reliable solution for protein concentration measurement in cell lysates, tissue extracts, and other biological samples. The included BSA protein standard enables convenient generation of standard curves for accurate protein quantification.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eLowry Protein Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eLowry Protein Quantification Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFolin-Ciocalteu Colorimetric Protein Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5–500 μg\/mL Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Wavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eA650 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetection Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e96-Well Microplate Reader or Spectrophotometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStandard\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBSA Protein Standard (5 mg\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein Quantification, Western Blot Sample Preparation, SDS-PAGE, Biochemical Research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBSA Standard: 2–8°C or -20°C; Other Reagents: Room Temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eClassical Lowry protein quantification method:\u003c\/strong\u003e Based on the widely used Folin-Ciocalteu chemistry for reliable total protein concentration measurement.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAccurate colorimetric protein detection:\u003c\/strong\u003e Generates a blue color signal proportional to protein concentration for quantitative analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWide protein detection range:\u003c\/strong\u003e Supports protein concentration measurement from 5–500 μg\/mL for common biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBSA protein standard included:\u003c\/strong\u003e Provides convenient standard curve preparation without requiring additional protein standards.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with multiple detection platforms:\u003c\/strong\u003e Suitable for microplate readers and conventional spectrophotometers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for routine protein analysis workflows:\u003c\/strong\u003e Supports protein quantification before Western blot, SDS-PAGE, and other downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eLowry Protein Assay Kit is widely used for total protein quantification in molecular biology, cell biology, and biochemical research workflows where accurate protein concentration measurement is required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Quantification:\u003c\/strong\u003e Determines protein concentration before SDS-PAGE loading to ensure consistent sample amounts across experimental groups.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Provides accurate protein measurement for electrophoresis experiments and protein profile analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Quantification:\u003c\/strong\u003e Measures total protein concentration from cultured cell extracts after protein extraction procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction Analysis:\u003c\/strong\u003e Supports protein concentration determination from tissue homogenates and biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP) and Co-IP:\u003c\/strong\u003e Helps normalize protein input amounts for antibody-based enrichment experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification Monitoring:\u003c\/strong\u003e Enables protein concentration evaluation during purification and biochemical characterization workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMicroplate Protein Assay:\u003c\/strong\u003e Suitable for high-throughput protein quantification using 96-well plate formats.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemistry Research:\u003c\/strong\u003e Used for routine total protein analysis and quantitative protein studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eThe Lowry protein assay procedure includes protein-copper complex formation followed by Folin-Ciocalteu reagent reduction and color development.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare Folin reagent working solution by mixing Folin reagent A and B at a 50:1 ratio.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDilute BSA protein standard to prepare a standard curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd standards and diluted samples into a 96-well plate or reaction tubes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Folin reagent working solution and incubate for color development.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Folin-Ciocalteu reagent and incubate at 37°C for approximately 30 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMeasure absorbance at A650 nm and calculate protein concentration based on the BSA standard curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause Lowry assays depend on amino acid composition and chemical interactions, samples containing phenolic compounds, citrate, or sulfhydryl-containing reagents may interfere with color development and should be evaluated before measurement.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0121\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eLowry Protein Assay Kit\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1000 Assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a Lowry Protein Assay Kit used for?\u003c\/summary\u003e\n\u003cp\u003eLowry Protein Assay Kit is used for quantitative measurement of total protein concentration in biological samples. It is commonly applied before Western blot, SDS-PAGE, immunoprecipitation, and biochemical protein analysis workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow does the Lowry protein assay work?\u003c\/summary\u003e\n\u003cp\u003eThe Lowry protein assay uses a two-step reaction. Proteins first react with copper ions under alkaline conditions to form protein-copper complexes. These complexes then reduce Folin-Ciocalteu reagent to generate a blue color signal proportional to protein concentration.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the detection range of this Lowry protein quantification kit?\u003c\/summary\u003e\n\u003cp\u003eThis Lowry Protein Assay Kit provides a protein detection range of 5–500 μg\/mL, making it suitable for common cell lysates, tissue extracts, and protein samples.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Lowry assay be used for Western blot sample preparation?\u003c\/summary\u003e\n\u003cp\u003eYes. Lowry protein quantification is commonly used before Western blot experiments to normalize protein loading amounts and improve experimental consistency.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat protein standard is included in the kit?\u003c\/summary\u003e\n\u003cp\u003eThe kit includes a BSA protein standard at 5 mg\/mL, which can be diluted to prepare a standard curve for protein concentration calculation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat substances interfere with Lowry protein assays?\u003c\/summary\u003e\n\u003cp\u003ePhenolic compounds, citrate, sulfhydryl-containing reagents, and differences in amino acid composition between proteins may affect color development and influence quantification accuracy.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Lowry protein assay be detected using a microplate reader?\u003c\/summary\u003e\n\u003cp\u003eYes. The assay is compatible with 96-well microplate detection using a plate reader at A650 nm, as well as measurement using a conventional spectrophotometer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"1000Assay","offer_id":52776488763573,"sku":"RP0121","price":77.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_d6eafc79-7d4e-4f05-ad90-c3be96ecf757.jpg?v=1783498180","url":"https:\/\/biofargo.com\/products\/lowry-protein-assay-kit","provider":"Biofargo","version":"1.0","type":"link"}