{"product_id":"lipoxidase-1","title":"Lipoxidase","description":"\u003carticle class=\"biofargo-product-detail\" lang=\"en\"\u003e\n\u003csection id=\"description\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLaboratory teams evaluating a Lipoxidase enzyme need clearly defined information about enzyme identity, biological source, physical form, solubility, activity, purity, storage, and assay conditions. Catalog No. BF-1980010 is Lipoxidase from soybean, identified as linoleate:oxygen oxidoreductase with Enzyme Commission number EC 1.13.11.12.\u003c\/p\u003e\n\u003cp\u003eThis soybean Lipoxidase is supplied in freeze-dried form and is readily soluble in distilled water or dilute buffer. Its listed activity is approximately 10 U\/mg protein, and its listed purity is approximately 60%. The product should be stored at −20°C (−4°F).\u003c\/p\u003e\n\u003cp\u003eLipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides. The supplied product information also provides a defined spectrophotometric activity method using linoleic acid at pH 9.0 and 25°C, with the change in absorbance measured at 232.5 nm.\u003c\/p\u003e\n\u003cp\u003eThe complete unit definition and assay conditions allow laboratories to interpret the stated Lipoxidase activity according to the method supplied with the product.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection id=\"specification\"\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003cdiv role=\"region\" style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\" tabindex=\"0\"\u003e\n\u003ctable style=\"width: 100%; min-width: 620px; border-collapse: collapse;\"\u003e\n\u003ccaption style=\"text-align: left; font-weight: 600; margin-bottom: 12px;\"\u003eSoybean Lipoxidase Product Specifications\u003c\/caption\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eProduct Name\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eLipoxidase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eSystematic Name\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eLinoleate:oxygen oxidoreductase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eEC Number\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eEC 1.13.11.12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eCatalog Number\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eBF-1980010\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eSource\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eSoybean\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eForm\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eFreeze-dried\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eSolubility\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eReadily soluble in distilled water or dilute buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eActivity\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eApproximately 10 U\/mg protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eApproximately 60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003e−20°C (−4°F)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eListed Price\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eUS$15.00\/g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eLipoxidase Unit Definition\u003c\/h3\u003e\n\u003cp\u003eOne unit will cause an increase in A232.5 of 0.001 per minute at pH 9.0 and 25°C when linoleic acid is the substrate in a 3.0 mL volume with a 1 cm light path.\u003c\/p\u003e\n\u003cp\u003eOne A232.5 unit is equivalent to the oxidation of 0.12 μmol of linoleic acid.\u003c\/p\u003e\n\u003cdiv role=\"region\" style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\" tabindex=\"0\"\u003e\n\u003ctable style=\"width: 100%; min-width: 620px; border-collapse: collapse;\"\u003e\n\u003ccaption style=\"text-align: left; font-weight: 600; margin-bottom: 12px;\"\u003eLipoxidase Activity Assay Conditions\u003c\/caption\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eSubstrate\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eLinoleic acid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eAssay Wavelength\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003e232.5 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eAssay pH\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003epH 9.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eAssay Temperature\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003e25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eReaction Volume\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003e3.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003eLight Path\u003c\/th\u003e\n\u003ctd style=\"padding: 12px; text-align: left; vertical-align: top; border: 1px solid #d9d9d9;\"\u003e1 cm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eAssay Reagents\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M ammonium hydroxide-ammonium chloride buffer, pH 9.0\u003c\/li\u003e\n\u003cli\u003e1 mM linoleic acid\u003c\/li\u003e\n\u003cli\u003eLipoxidase diluted to 0.6 U\/mL\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eAssay Procedure\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eSet the spectrophotometer, equipped with a strip chart recorder and temperature control, to 232.5 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003ePipette 2.9 mL of ammonium hydroxide-ammonium chloride buffer into the quartz cuvettes.\u003c\/li\u003e\n\u003cli\u003eAdd 0.1 mL of the enzyme solution to the test cuvette, mix, and record the rate of absorbance at 232.5 nm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate ΔE232.5 nm\/min from the initial linear portion of the curve.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eActivity Calculation\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eActivity (U\/mg) =\u003c\/strong\u003e (ΔE232.5 nm\/min × Total Vol. × Enz. Diln.) ÷ (27.4 × Enz. Vol. × mg Enz.\/mL)\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection id=\"features\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLipoxidase from soybean\u003c\/li\u003e\n\u003cli\u003eLinoleate:oxygen oxidoreductase\u003c\/li\u003e\n\u003cli\u003eEnzyme Commission number EC 1.13.11.12\u003c\/li\u003e\n\u003cli\u003eCatalog number BF-1980010\u003c\/li\u003e\n\u003cli\u003eSupplied in freeze-dried form\u003c\/li\u003e\n\u003cli\u003eReadily soluble in distilled water or dilute buffer\u003c\/li\u003e\n\u003cli\u003eActivity of approximately 10 U\/mg protein\u003c\/li\u003e\n\u003cli\u003ePurity of approximately 60%\u003c\/li\u003e\n\u003cli\u003eStorage at −20°C (−4°F)\u003c\/li\u003e\n\u003cli\u003eDefined spectrophotometric assay at 232.5 nm\u003c\/li\u003e\n\u003cli\u003eDefined activity assay using linoleic acid at pH 9.0 and 25°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"application\"\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eLipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides.\u003c\/p\u003e\n\u003cp\u003eThe supplied product description states that the degradation of the hydroperoxide by Lipoxidase appears to prevent the breakdown of amino acids and proteins associated with an odorous carbonyl compound that produces the beany flavor found in many legumes.\u003c\/p\u003e\n\u003cp\u003eThe supplied information states that Lipoxidase is widely distributed and is especially concentrated in legumes, with soybeans having the highest concentrations.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection id=\"faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Lipoxidase Catalog No. BF-1980010?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eCatalog No. BF-1980010 is Lipoxidase from soybean. Its systematic name is linoleate:oxygen oxidoreductase, and its EC number is EC 1.13.11.12.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the source of this Lipoxidase?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe source of Lipoxidase Catalog No. BF-1980010 is soybean.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the EC number of Soybean Lipoxidase?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe Enzyme Commission number listed for this Soybean Lipoxidase is EC 1.13.11.12.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the systematic name of this Lipoxidase enzyme?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe systematic name stated in the supplied product information is linoleate:oxygen oxidoreductase.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the physical form of Lipoxidase BF-1980010?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eLipoxidase Catalog No. BF-1980010 is supplied in freeze-dried form.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs Soybean Lipoxidase soluble in water?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe product is readily soluble in distilled water or dilute buffer.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the activity of this Soybean Lipoxidase?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe listed activity is approximately 10 U\/mg protein.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs 10 U\/mg protein a minimum guaranteed activity?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eNo minimum value is stated in the supplied product information. The activity is listed as approximately 10 U\/mg protein.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the purity of Lipoxidase BF-1980010?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe listed purity is approximately 60%.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs 60% a minimum guaranteed purity?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eNo minimum value is stated in the supplied product information. The purity is listed as approximately 60%.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should Soybean Lipoxidase be stored?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe stated storage condition is −20°C, equivalent to −4°F.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow is one Lipoxidase activity unit defined?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eOne unit will cause an increase in A232.5 of 0.001 per minute at pH 9.0 and 25°C when linoleic acid is the substrate in a 3.0 mL volume with a 1 cm light path.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat does one A232.5 Lipoxidase unit represent?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eOne A232.5 unit is equivalent to the oxidation of 0.12 μmol of linoleic acid.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat substrate is used in the Lipoxidase activity assay?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eLinoleic acid is used as the substrate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAt what wavelength is Lipoxidase activity measured?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe activity method measures the change in absorbance at 232.5 nm.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat pH and temperature are used in the Lipoxidase assay?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe assay is performed at pH 9.0 and 25°C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat reaction does Lipoxidase catalyze?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eLipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat price is listed for Lipoxidase BF-1980010?\u003c\/summary\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe supplied product information lists a price of US$15.00 per gram.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c\/article\u003e","brand":"calzyme","offers":[{"title":"1 g","offer_id":52981795324085,"sku":"BF-1980010-1","price":0.0,"currency_code":"USD","in_stock":true},{"title":"10 g","offer_id":52981795356853,"sku":"BF-1980011-10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"100 g","offer_id":52981795389621,"sku":"BF-1980012-100","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Lipoxidase_BF-1980010_1500x1500_6c066921-98f5-4ff7-b0fc-2ec8fc275431.jpg?v=1787026333","url":"https:\/\/biofargo.com\/products\/lipoxidase-1","provider":"Biofargo","version":"1.0","type":"link"}