{"product_id":"lipoprotein-depleted-fetal-bovine-serum","title":"Lipoprotein-Depleted Fetal Bovine Serum (LPDS)","description":"\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLipoprotein-Depleted Fetal Bovine Serum (LPDS) is fetal bovine serum from which lipoproteins have been removed by density ultracentrifugation. Removing LDL, HDL and VLDL takes away the main external source of cholesterol and other lipids that cultured cells normally take up from serum, while keeping the serum proteins and growth-supporting factors.\u003c\/p\u003e\n\u003cp\u003eCells grown in LPDS must rely on their own cholesterol synthesis and lipoprotein uptake pathways. This makes LPDS the standard serum supplement for studying cholesterol biosynthesis, SREBP signaling, LDL receptor regulation and lipoprotein uptake. After ultracentrifugation, the serum protein concentration is adjusted to approximately 35 mg\/mL with phosphate-buffered saline (PBS), and the product is sterile-filtered, aseptically packaged and frozen.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLipoprotein-Depleted Fetal Bovine Serum (LPDS)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCatalog Numbers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBF88307169 (10 mL) \/ BF96470191 (50 mL) \/ BF53981708 (100 mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSource\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFetal bovine serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eLipoprotein Removal\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDensity ultracentrifugation (lipoproteins with d \u0026lt; 1.25 g\/mL removed)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein Concentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e≥ 35 mg\/mL, adjusted with PBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eLipoprotein Depletion Check\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAgarose gel electrophoresis with lipid staining\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterility\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterile-filtered; aseptically packaged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eForm\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFrozen liquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003ePack Sizes\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 mL, 50 mL, 100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e≤ −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended Use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLipoproteins removed by ultracentrifugation\u003c\/strong\u003e — the reference method for preparing lipoprotein-deficient serum.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eSerum proteins retained\u003c\/strong\u003e and adjusted to about 35 mg\/mL, so cells stay viable and attached in lipid-depleted conditions.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eRemoves exogenous cholesterol\u003c\/strong\u003e, activating SREBP-2 and LDL receptor expression in cultured cells.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLot-tested for depletion\u003c\/strong\u003e by agarose gel electrophoresis with lipid staining.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eThree pack sizes\u003c\/strong\u003e (10, 50 and 100 mL) for pilot work through larger studies.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eCholesterol Biosynthesis:\u003c\/strong\u003e Study the mevalonate pathway and HMG-CoA reductase regulation under sterol-depleted conditions.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eSREBP Signaling:\u003c\/strong\u003e Induce SREBP-2 processing and target gene expression.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLDL Receptor and PCSK9 Research:\u003c\/strong\u003e Up-regulate LDL receptor expression for LDL binding and uptake assays.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLipoprotein Uptake:\u003c\/strong\u003e Measure uptake of labeled LDL, HDL or VLDL without competition from serum lipoproteins.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Evaluate statins and other lipid-lowering compounds in sterol-depleted cells.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLipid Metabolism in Cancer and Immunology:\u003c\/strong\u003e Examine how tumor and immune cells depend on exogenous lipids.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003eStore at ≤ −20 °C. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eThaw at 2–8 °C or at room temperature, mix gently, and aliquot for single use.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eShipped on dry ice.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\u003cp\u003eThaw the serum at 2–8 °C overnight or at room temperature; avoid prolonged warming at 37 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eMix gently by inversion and aliquot to avoid repeated freeze–thaw.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eReplace regular FBS with LPDS in the culture medium, typically at 5–10% (v\/v). The optimal concentration and exposure time depend on the cell type and assay.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eFor sterol-depletion protocols, culture cells in LPDS-containing medium for 16–24 hours before stimulation or analysis; statins can be added to further suppress endogenous cholesterol synthesis.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n  .bf-rich .josh-faq { background: #fff; color: #000; font-family: \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n  .bf-rich .josh-faq__question { width: 100%; margin: 0; padding: 20px 0; display: flex; align-items: center; justify-content: space-between; gap: 16px; color: #505052; background: none; border: 0; font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif; text-align: left; cursor: pointer; list-style: none; }\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #505052; }\n  .bf-rich .josh-faq__icon { position: relative; width: 20px; height: 20px; flex: 0 0 20px; transition: transform .25s ease; }\n  .bf-rich .josh-faq__icon::before, .bf-rich .josh-faq__icon::after { position: absolute; content: \"\"; background: currentColor; border-radius: 2px; transition: opacity .25s ease, transform .25s ease; }\n  .bf-rich .josh-faq__icon::before { top: 50%; left: 0; width: 100%; height: 2px; transform: translateY(-50%); }\n  .bf-rich .josh-faq__icon::after { top: 0; left: 50%; width: 2px; height: 100%; transform: translateX(-50%); }\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after { opacity: 0; transform: translateX(-50%) rotate(90deg); }\n  .bf-rich .josh-faq__answer { max-width: 860px; padding: 0 0 20px; color: #505052; font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #505052; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n  @media (max-width: 767px) { .bf-rich .josh-faq__inner { padding: 24px 0 40px; } .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; } .bf-rich .josh-faq__answer { font-size: 15px; } }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cp\u003eCommon questions about Lipoprotein-Depleted Fetal Bovine Serum (LPDS).\u003c\/p\u003e\n\u003cdiv class=\"josh-faq\"\u003e\u003csection class=\"josh-faq__inner\"\u003e\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat is lipoprotein-depleted serum used for?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eIt is used to culture cells without exogenous lipoproteins, for studies of cholesterol synthesis, SREBP signaling, LDL receptor regulation, lipoprotein uptake and lipid-lowering drugs.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow are the lipoproteins removed?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eLipoproteins are removed by density ultracentrifugation, then the serum protein concentration is adjusted to about 35 mg\/mL with PBS.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan LPDS completely replace FBS in my medium?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. LPDS is used in place of regular FBS, usually at 5–10%. Some cell types grow more slowly in lipid-depleted conditions, so check viability for your cells.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eIs lipoprotein-depleted serum the same as delipidated or charcoal-stripped serum?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. LPDS has lipoproteins removed by ultracentrifugation. Delipidated serum is solvent-extracted to remove lipids, and charcoal-stripped serum mainly removes hormones and other small lipophilic molecules.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow should LPDS be stored?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eStore at −20 °C or below and aliquot after the first thaw to avoid repeated freeze–thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan it be used for clinical or diagnostic purposes?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What is lipoprotein-depleted serum used for?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It is used to culture cells without exogenous lipoproteins, for studies of cholesterol synthesis, SREBP signaling, LDL receptor regulation, lipoprotein uptake and lipid-lowering drugs.\"}}, {\"@type\": \"Question\", \"name\": \"How are the lipoproteins removed?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Lipoproteins are removed by density ultracentrifugation, then the serum protein concentration is adjusted to about 35 mg\/mL with PBS.\"}}, {\"@type\": \"Question\", \"name\": \"Can LPDS completely replace FBS in my medium?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. LPDS is used in place of regular FBS, usually at 5–10%. Some cell types grow more slowly in lipid-depleted conditions, so check viability for your cells.\"}}, {\"@type\": \"Question\", \"name\": \"Is lipoprotein-depleted serum the same as delipidated or charcoal-stripped serum?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. LPDS has lipoproteins removed by ultracentrifugation. Delipidated serum is solvent-extracted to remove lipids, and charcoal-stripped serum mainly removes hormones and other small lipophilic molecules.\"}}, {\"@type\": \"Question\", \"name\": \"How should LPDS be stored?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Store at −20 °C or below and aliquot after the first thaw to avoid repeated freeze–thaw cycles.\"}}, {\"@type\": \"Question\", \"name\": \"Can it be used for clinical or diagnostic purposes?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\"}}]}\u003c\/script\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/biofargo.com\/pages\/contact\"\u003e\u003cstrong\u003eRequest Datasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n","brand":"Biofargo","offers":[{"title":"10 mL","offer_id":53415502151861,"sku":"BF88307169","price":159.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":53415502184629,"sku":"BF96470191","price":499.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":53415502217397,"sku":"BF53981708","price":839.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/lipoprotein-depleted-fetal-bovine-serum","provider":"Biofargo","version":"1.0","type":"link"}