Description

Lewis Rat Primary Cardiac Fibroblasts from Cell Biologics are isolated from the heart tissues of 6–8-week-old laboratory Lewis rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Lewis Rat Primary Cardiac Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-Vimentin antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Suggested Medium

M2267 Complete Fibroblast Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6049LS

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.