{"product_id":"lewis-rat-primary-aortic-endothelial-cells","title":"Lewis Rat Primary Aortic Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLewis Rat Primary Aortic Endothelial Cells from Cell Biologics are isolated from the aortic tissues of Lewis rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Rat Primary Aortic Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM1266 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6052LS_RA-6052LS.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eRA-6052LS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6052LS_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481672904885,"sku":"RA-6052LS","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481672937653,"sku":"RA-6052LS-1","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flasi","offer_id":52481672970421,"sku":"RA-6052LS-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rat_EC.Tube_formation1-500x500.jpg?v=1778272563","url":"https:\/\/biofargo.com\/products\/lewis-rat-primary-aortic-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}