{"product_id":"kod-flex-pcr-master-mix","title":"KOD FLEX PCR Master Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eKOD FLEX™ PCR Master Mix is a highly efficient and high-success-rate 2× PCR master mix. It incorporates a modified KOD DNA polymerase (UKOD), an elongation accelerator, and an unique buffer technology to enable high-efficiency, low-bias amplification of hard-to-amplify targets.\u003c\/p\u003e\n\u003cp\u003eEfficient amplification is possible even for hard-to-amplify targets such as high GC\/AT content regions, long DNA fragments, and those with low amplification specificity. Amplification is possible even from crude samples such as biological samples.\u003c\/p\u003e\n\u003cp\u003eThis master mix demonstrates approximately 11 times the fidelity of Taq DNA polymerase, making the resulting amplified products suitable for conventional sequencing analysis via cloning. Furthermore, it is characterized by reduced amplification bias related to sequence composition or amplicon size, making it suitable for library amplification in next-generation sequencing.\u003c\/p\u003e\n\u003cp\u003eKOD FLEX™ PCR Master Mix contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and its 3ʹ→5ʹ exonuclease activity, thus allowing for Hot Start PCR. Furthermore, KOD FLEX™ PCR Master Mix generates blunt-end PCR products because of its 3ʹ→5ʹ exonuclease (proof-reading) activity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHigh success-rate・High elongation efficiency\u003c\/strong\u003e\u003cbr\u003eOriginal buffer composition enables high specificity. PCR efficiency is improved by adding an “Elongation Accelerator”. The extension rate can be reduced by decrements of up to 15 s\/kb in singleplex PCR. (Note that to realize sufficient amplification, the extension rate of ~30–60 s\/kb is recommended for amplifications of crude samples or for the multiplexed amplification of long targets.)\u003cbr\u003e\u003cbr\u003eThe cycling conditions can be set flexibly when various targets having different sizes are amplified.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eApplicable for amplification from crude samples\u003c\/strong\u003e\u003cbr\u003eKOD FLEX™ PCR Master Mix is effective for amplification from crude samples (e.g. biological samples, foodstuffs, soil extract, etc) because of its tolerance of the PCR inhibitors commonly present in crude samples.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHomogeneous amplification (Low bias)\u003c\/strong\u003e\u003cbr\u003eHomogeneous multiplex PCR of targets up to 10 kb can be achieved. Various regions of the genome or transcriptome can be amplified homogeneously even if these regions contain GC bias. The resulting amplicons are also suitable for next-generation sequencing analyses.\u003cbr\u003eEffect of GC bias on amplification is minimized, enabling uniform amplification regardless of the target. This master mix is suitable for library amplification for next-generation sequencers of long reads as well as short reads due to low bias and high elongation.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHigh fidelity\u003c\/strong\u003e\u003cbr\u003eKOD FLEX™ PCR Master Mix exhibits approximately 11-fold higher fidelity than Taq DNA polymerase, so this master mix can accurately amplify long targets. The amplified products can be used for various purposes, including next-generation sequencing analysis as well as cloning.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Primers containing uracil (U) or Inosine (I) can be used\u003cbr\u003eKOD FLEX™ PCR Master Mix can use primers containing inosines (I) or uracil (U), whereas conventional high-fidelity PCR enzymes cannot. Bisulfite-treated targets and degenerate primers can be used.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_fidelity_0.png?v=1775546121\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003e・Genotyping (Amplification from crude sample)\u003cbr\u003eDirect amplification from;\u003cbr\u003e-Whole blood, cultured cell, mouse tail, mouse toe , plant and food stuff etc.\u003cbr\u003e-Various kinds of lysate from mouse tail, plant, fish fin, soil etc.\u003cbr\u003e- Gram-positive bacteria, fungus, yeast etc.\u003cbr\u003e\u003cbr\u003e・Efficient amplification of difficult targets (high G\/C, long ) with fast mode.\u003cbr\u003e\u003cbr\u003e・High fidelity PCR\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left; width: 25.7261%;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left; width: 74.1701%;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc; width: 25.7261%;\"\u003eSource\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; width: 74.1701%;\"\u003eE. coli strain carrying the cloned UKOD DNA polymerase gene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; width: 25.7261%;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; width: 74.1701%;\"\u003eStore at -20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eKOD FLEX™ PCR Master Mix includes the following components for 200 reactions, 50 μl total reaction volume.\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left; width: 25.7261%;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left; width: 74.1701%;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc; width: 25.7261%;\"\u003e2x KOD FLEX™ PCR Master Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; width: 74.1701%;\"\u003e1 mL x 5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Amplification of long targets\u003c\/h3\u003e\n\u003cp\u003eLong targets were amplified using human genomic DNA as template.\u003cbr\u003eEven extremely long targets of up to 40 kb were successfully amplified with KOD FLEX™ PCR Master Mix.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application1_1.png?v=1775546204\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Amplification of GC\/AT-rich targets\u003c\/h3\u003e\n\u003cp\u003eUsing human genomic DNA as template, GC-rich (approximately 70% G+C) and AT-rich (approximately 30% A+T) regions were amplified.\u003cbr\u003eKOD FLEX™ PCR Master Mix demonstrated stable amplification of regions with high GC content as well as of regions with high AT content.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application2_0.png?v=1775546283\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Amplification from crude samples (blood)\u003c\/h3\u003e\n\u003cp\u003eThe WT-1 gene (2.5 kb) was amplified using blood as template.\u003cbr\u003eUsing KOD FLEX™ PCR Master Mix, amplification was achieved even when 30% blood was added to the reaction mixture, without any inhibitory effects from the blood.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application3_0.png?v=1775546303\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Amplification of various targets\u003c\/h3\u003e\n\u003cp\u003eEight different targets were amplified using human genomic DNA as template.\u003cbr\u003eWhile no amplification or non-specific amplification was observed using other companies' reagents, good amplification was observed when using KOD FLEX™ PCR Master Mix.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application4_0.png?v=1775546328\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 5.Multiplex PCR\u003c\/h3\u003e\n\u003cp\u003eMultiplex PCR was performed using human genomic DNA as template. The number of multiplex targets was gradually increased, starting from short fragments, to evaluate both reaction efficiency and specificity.\u003cbr\u003eWhile some targets failed to amplify with other companies' reagents, KOD FLEX™ PCR Master Mix successfully produced bands corresponding to the intended number of multiplex targets.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application5_0.png?v=1775546352\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 6.NGS library amplification\u003c\/h3\u003e\n\u003cp\u003eGenomic NGS libraries of E.coli (approximate GC content: 51%) and Thermus thermophilus (Tth, approximate GC content: 70%) were prepared, and those amplified with KOD FLEX™ PCR Master Mix for 7 cycles were compared with PCR-free libraries. Sequencing was performed using an Illumina MiSeq™ System.\u003cbr\u003eCoverage comparisons across a range of GC contents showed no significant differences between PCR-free and amplified libraries, indicating that the libraries were successfully amplified without any bias.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_application6_0.png?v=1775546349\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_manual_EN.pdf?v=1775806692%20\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX-101_SDS_EN.pdf?v=1775806689\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KMX_flyer_EN.pdf?v=1775806843\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\"\u003e Download Flyer \u003c\/a\u003e \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"200Rxn","offer_id":52417750335669,"sku":"KMX-101","price":250.32,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/kod-flex-pcr-master-mix","provider":"Biofargo","version":"1.0","type":"link"}