{"product_id":"immortalized-rfp-expressing-human-primary-carotid-artery-endothelial-cells","title":"Immortalized RFP-Expressing Human Primary Carotid Artery Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eImmortalized RFP-Expressing Human Primary Endothelial Cells from Cell Biologics are isolated from human tissue and grown in gelatin pre-coated tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized RFP-Expressing Human Primary Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), or VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\n\u003cp\u003e Immortalized cell lines from Cell Biologics are derived from primary cells expressing RFP. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\n\u003cp\u003e Red Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168                Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"0.5x10^6 Cells (Frozen Vial)","offer_id":52481501298869,"sku":"H-6008IM.RFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/no_image-500x500_5c011fa4-6a68-47a6-9e13-c5a09e770e26.webp?v=1778271272","url":"https:\/\/biofargo.com\/products\/immortalized-rfp-expressing-human-primary-carotid-artery-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}